LOCUS       BG071848                 609 bp    mRNA    linear   EST 26-JAN-2001
DEFINITION  H3103H02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3103H02 3', mRNA sequence.
ACCESSION   BG071848
VERSION     BG071848.1
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
            Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
  TITLE     Verification and initial annotation of NIA mouse 15K cDNA clone set
  JOURNAL   Unpublished
COMMENT     Other_ESTs: H3103H02-5
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3103  row: H  column: 02
            Seq primer: -21M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3103H02-3"
                     /db_xref="taxon:10090"
                     /clone="H3103H02"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          162 a          199 c           82 g          163 t
ORIGIN      
        1 acacaggggg gggagtatat tctcgattat aaataacctt tacaggcaaa catctctcaa
       61 actaaaacca atcctttgat cggattaccc atttttccca ccacttcatc cccttcaccc
      121 ataacattct gccccagcat cccacccaac catcacatct tcactcactc actctgtaga
      181 cttcacacct tcaatttcac tttgttctcc tagctcatga attactcctc ccttccacag
      241 tcacctgacc tcgtatcctc ctgtatgatt gccatctcgg acatctacag cataaattga
      301 aataacccaa ttaaaagagc ctctttctac atcctgcatc tcattaccnt catcgagctc
      361 actagaccac tgattacaga ataccctgta tagtccatct acccagccct tacctagtag
      421 aacatccaac tccctcgctt catactagat tttgatgnct ccaacggcta cagcttactt
      481 tgctcacatc gtggcctaag gtacanggtg tgcccttaaa ctgcaggcct ggaacagact
      541 ccacctcctc tgaccgttca ccatgcgctt tgcctagaaa ggagacacca gacggatact
      601 cgacccgta
//