LOCUS BG071766 744 bp mRNA linear EST 12-MAY-2010
DEFINITION H3103A01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3103A01 3', mRNA sequence.
ACCESSION BG071766
VERSION BG071766.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 744)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG071766.1.
Other_ESTs: H3103A01-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3103 row: A column: 01
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..744
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3103A01-3"
/db_xref="taxon:10090"
/clone="H3103A01"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 199 a 167 c 164 g 214 t
ORIGIN
1 aaagagactc cattattatc tgcaccttct ataggaggca aaatgatgaa tctggagcta
61 gggacactct tgggtggtag aaggctcagc tagctcgctt gaagccagag cgtgcattcc
121 taacactgca tacaccggat acggcagtgt ggggccttaa ctgggaatct gagagatggc
181 aagtgccagg ccatcctcag ctccatggtg aacctgtggc cagcctctgc tgcatgagac
241 accctctaac gcaaacaaaa ggaactacag tagcaaaatt attaatctta actagtgatc
301 tgatgatgtg tccaggttcc cctctgtttt cctttgtatt tcatgattat tatttctgta
361 ttttctctgt ttgattttat tgtagtaaaa ctctgttata gggtaagcta agccattttc
421 tcttcctcat ttaatttctg gagttaacct tcctagtctt atttaggatt tgttttttaa
481 ggatacagaa atgttgtgtc cttaaacaaa caaacaaaca aaacccagca ttcaaaaggc
541 cacgctgggc gtctgttgtg tggatttacc tccaagctgt ggaaggacac atgaagagaa
601 aaccttccga aatggacgat ggggctcacc tttcactcca gtgagcatct agcacagagc
661 tacctatggg tgtcacatct gcccacatgc tctttaatcc tgagatccta ggaagccatt
721 actgtcggtc ggcacaggct ggag
//