LOCUS BG071211 431 bp mRNA linear EST 12-MAY-2010
DEFINITION H3096A04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3096A04 3', mRNA sequence.
ACCESSION BG071211
VERSION BG071211.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 431)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT Other_ESTs: H3096A04-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3096 row: A column: 04
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..431
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3096A04-3"
/db_xref="taxon:10090"
/clone="H3096A04"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 92 a 125 c 94 g 120 t
ORIGIN
1 aagttttcca tgtctacatc taattgatta agttaggtct tcacaccagg tagctcagct
61 tcacgtccag gctagcaagg taggtccctt gtcctggtct cacagaggtc aatgccggcc
121 tgcagcttgc cattcttcac cgctgcccag ggcatggagg cagaggtcca ctttgccacc
181 cagtctcctg ttttgttgac caagatgagg ccacctagac ctttgagttt tgacttcatg
241 taatccaatg ccaactgagc agcctcctct acggcctttc cttgctctac atggaagagg
301 gcaagtctgg ccagattcac cttcaggata ctttccccat gtcctgtggt tgaaacggct
361 ccaaggttat tatctgcgta acctccagct cctatgcaag gcgagtctcc aactcggcca
421 accattttat t
//