LOCUS       BG071100                 607 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3094F11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3094F11 3', mRNA sequence.
ACCESSION   BG071100
VERSION     BG071100.2
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG071100.1.
            Other_ESTs: H3094F11-5
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3094  row: F  column: 11
            Seq primer: -21M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3094F11-3"
                     /db_xref="taxon:10090"
                     /clone="H3094F11"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          131 a          174 c          160 g          142 t
ORIGIN      
        1 ctttgtaaaa ggcacggttt ttattgaaac aaggccgcat tgctctctga ggactcaact
       61 ccccttggct gcaacacgca tccctgctgg attctggaca cccacccact cccctttttg
      121 actccttccc agccggccac ttgtcagaca gagtgctcat atggaaaagg gcactagaaa
      181 tccacaagtg agctgtgaca gcacaggggc tacgtggagg tggttctaca gcaccaaccc
      241 gacgagcctg gggacccgat gaccccgatc ccagcatctc taaggcagag ctctggcctc
      301 aggaccatgt ctcagttact tcactggcaa gtgaccagct ccttggaggg tgagttcatg
      361 tttgagacat ggggctaaag gtgatgtcct cacacctcac gaggctgtgg ctgtggcggc
      421 attgggttgg accgaagctt ctggtgccct accgcctctg cttgggtcag gggttgcctg
      481 acacctggct gcttgcgtta tatgcccaca cgtgccatgg ctcatctatg ctttcaacag
      541 aaccacagaa ctttccagaa actccagggt tctgtgaggt tggaaccaca gcagtcatta
      601 tctgggg
//