LOCUS BG070917 730 bp mRNA linear EST 26-JAN-2001
DEFINITION H3091G01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3091G01 3', mRNA sequence.
ACCESSION BG070917
VERSION BG070917.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 730)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Other_ESTs: H3091G01-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3091 row: G column: 01
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..730
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3091G01-3"
/db_xref="taxon:10090"
/clone="H3091G01"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 192 a 189 c 166 g 183 t
ORIGIN
1 ggttgttttg tttccttttt ttttccgaga caatgtccca ccttgtagct atggttggtc
61 tgattagaca ggctggccta gaactcaaga gatccagctg cccctgagat taaaggcatg
121 caccagcaca gcaggcttgt ttttgttttg aggcagggtc tgactacata gtctcagctg
181 gcctgtttgg gagcttctat agacgagact cgtctagctc ggcactccct gacttgcctc
241 tgagactgag catctgctac catgcccagc ggccactctt gttggcaaga tgaccatgtt
301 gccaatatat gtatgcttat actcagacca cagctaccct caaccttcat ctaagacatt
361 tctagccatg gcagcattaa cacagactca tatctaatca aaatactaag aatgagtagc
421 tgttgagaaa ccagccctac acaagcacat ctatgttacc caactcttcc cagagttcag
481 aggacatcct cgaagagtaa gggtagaaag aattataagt gtgggctaca gagatggctc
541 agaggttaag agcactggct gtctacagaa ggccccaggt tcagctgttc ttcatgagga
601 cccaggctca attcccagca tcacagggag caccccacta aactaaccct cccacacccc
661 gctttggcat agtcagcacc aggcatgaga gtggtgcaca gttcacggga atcagtgaat
721 ggatagtcga
//