LOCUS BG070466 813 bp mRNA linear EST 26-JAN-2001
DEFINITION H3087F07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3087F07 3', mRNA sequence.
ACCESSION BG070466
VERSION BG070466.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 813)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Other_ESTs: H3087F07-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3087 row: F column: 07
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..813
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3087F07-3"
/db_xref="taxon:10090"
/clone="H3087F07"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 228 a 226 c 142 g 217 t
ORIGIN
1 acatttccat actgctgttg atcaccaaag gatgcaggac tggaactcaa gcaggtcaga
61 aagcaggagc tgatgcagag gccatggagg gatgttcttt actggcttgc tcagcctgct
121 ctcttataga acccaagaca accagcccag agatggtccc acccacaagg ggccttgccc
181 ctgtgatcac taattgagaa aatgccttac agttggatct catggaggca tttcctcaac
241 tgaagctcct ttctctgtga taactccagc agtgtcaagt tgacacaaaa ctagccaata
301 caactgaccc cttgtcaact tgacacacaa acacatcact agtaagcctc aacccttaca
361 ttcttattca tccccaaggt ctaaataact ttaaatgtcc cacagtcttt acatattctt
421 aaaatttcaa tctctttaaa atatccatct cttttaaaaa tccaaagtct ttttaaatta
481 aaagtctctt aactgtgggc atcactaaaa cagtttcttc cttcaagagg gaaaatatca
541 gggcacagtc acaatcaaaa gcaaaaatca atctccaacc gttcaatgtc tgggatccaa
601 ctcacgatct tctgggctcc tccaagggct tgggtcactt ctccagccat gccctttgta
661 gcacacgcgt catcctctag gctccagatg cctctactcc actgctgctg ctgctcttgg
721 tggtcatctc atggtactgg catctccaaa acactgcatg accccttcag tcctgggcca
781 tcaattgcaa ctgaggctgc actttcacca atg
//