LOCUS BG070363 809 bp mRNA linear EST 12-MAY-2010
DEFINITION H3086E09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3086E09 3', mRNA sequence.
ACCESSION BG070363
VERSION BG070363.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 809)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG070363.1.
Other_ESTs: H3086E09-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3086 row: E column: 09
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..809
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3086E09-3"
/db_xref="taxon:10090"
/clone="H3086E09"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 186 a 218 c 173 g 232 t
ORIGIN
1 gaaaaggtaa tgtgctataa tgatattcgt ttgcaacatc aaaagcagca gaaatactga
61 atataatttg attaaatcat aaatactgag attgactatg cacatagaaa ttacatttag
121 gctccatgag ctgagagctc tctaaaggag atgaggtgcg aaccagcgag atcagctcct
181 ggcccatcaa tactcccact cgtccgactt catgtccagg tagctgagga tgttctgggc
241 cagtttcaca gcctgtttcc tggcagcctt acacttctct tctccctgtg ggtcaacggc
301 atccagagcc agcaactgtt tggtcagtag ctcctccaga cggatgtagt tcttatcagt
361 tcggtttcca ccaaatgaaa ggacttcgcc caggatctca gacaggttcc caaggatctc
421 ccacacggcc ttgtgtgggg ggtgctcctc acatgggaac agcttcctct tctcgagggc
481 ttccttcagg tccaggtacg tgatcaggat ctgcacctca atcaccgccc tcctcctggc
541 ctcccggatg caggggtttt tctccagact cacttcatcc agctgtccga tcaagccctg
601 cagctctgtc ttggccctca ggtacaactc aggagggctc tgcgcctgga gaagttcatt
661 ttttatctct ctcatcctct tgagaacatt ttctattttt ataatggaat ggttctgtcc
721 caggtcaaat gcatgtgtac tgtcagcttc ctcttctaag tctaggtatt tcagtaattt
781 gttgatatct tccaccacct ccctccgat
//