LOCUS BG069401 750 bp mRNA linear EST 12-MAY-2010
DEFINITION H3076H10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3076H10 3', mRNA sequence.
ACCESSION BG069401
VERSION BG069401.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 750)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG069401.1.
Other_ESTs: H3076H10-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3076 row: H column: 10
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..750
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3076H10-3"
/db_xref="taxon:10090"
/clone="H3076H10"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 173 a 184 c 178 g 215 t
ORIGIN
1 ccttcaagtt tttgtttggt tggttggttt tattggcaag catcagtgcc gcattaccgt
61 cgattcccca ggcttcatcg tgctgcggtg agaccactgg aaagcacggt gttctcagca
121 caaggctggg actgctcctt caccaccggg tagaaatatg ggtgctccat ggcctctttg
181 gcggtcaatc tctgttgatg gtcgtaccgc agagcttgtc aagaagatct agggcctccg
241 ggctgacaag gtgcctgttc tcactatgga taaagttttc ccagcgcttc cgtgaatgtt
301 gtcccaggat atcattgaag tgtggatcta ggtctatgtg gtacttcttc agataaccat
361 atagttcatc tgtccccaga accttggcaa ttcgaacaag ctggtcatag ttgtcctgcc
421 cgtggaagaa tggctccttt cggaatatca tgctcgctaa catgcagccc aagctccaca
481 tgtccaagct ataatcatac atctgatagt ccacaaggag ctctggtccc ttgaagtacc
541 tcgaggccac tcgaacattg tactcctgag caggatgata gaactctgcc agaccccaat
601 caatcagtcg gagctttttt tgttggtgat ctatcatgac attgtgaggt ttcacatccc
661 tgtgcatgat tcccttgctg tggcagtaat ccagagcttt aagtagttca tacatataaa
721 accggatatc aaagtcagtc aggatctggt
//