LOCUS BG069374 491 bp mRNA linear EST 12-MAY-2010
DEFINITION H3076F06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3076F06 3', mRNA sequence.
ACCESSION BG069374
VERSION BG069374.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 491)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG069374.1.
Other_ESTs: H3076F06-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3076 row: F column: 06
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..491
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3076F06-3"
/db_xref="taxon:10090"
/clone="H3076F06"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 149 a 80 c 155 g 107 t
ORIGIN
1 aacaataaaa tagctctttt tttattcact ttgatttgaa taattggaaa tattcaacaa
61 taaataaaat ggagccgagg ctgaggaagg cagactccct gacatcattg gaaatgcatt
121 cagaccaatc aggaggatta caagacgtgg gcaggagaaa gcaaagttta aacaggcaat
181 gctgggtcac aggaggcaag gaaggaagag ctttcgggca gaaagtgctt ggaaaacttt
241 ggctcggaag cagacttggg aaagggctaa agtgattgtg cctagaggtg caggaggaac
301 ccatgtctac ctactagggt gggttgagca aagtccagat caggctcttg ggaaatggtt
361 aaagtgattg tgcaaggagg tggggggcag caggagttac ccatgttcat ctggtagggt
421 gtgcaagatc cggattaggc tctggagaaa gggttaaagc ggttgccatg gggcagaccc
481 tgggcaggcg g
//