LOCUS BG068881 564 bp mRNA linear EST 12-MAY-2010
DEFINITION H3070C11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3070C11 3', mRNA sequence.
ACCESSION BG068881
VERSION BG068881.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 564)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG068881.1.
Other_ESTs: H3070C11-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3070 row: C column: 11
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..564
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3070C11-3"
/db_xref="taxon:10090"
/clone="H3070C11"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 147 a 145 c 122 g 150 t
ORIGIN
1 atcagcatca ggtcaccagg cagcaactat gagctacaaa ctctaaaggt ctgaataggc
61 agacagggaa agcgaggtct ctcttcttcc ttgagcatgt gcaagcccct ctgcacacac
121 agacttatcc atgttgcatc ctcctggggg aggcaggttt gcaaacacat cctgtgacac
181 cacttggggt gaatgcggtg ctgtaataaa gccataaaac cccatgggtc gaagcccctc
241 tgtgatcact gtcccagatg cccctgcttt ctttctttct tgtgtgttgt tcaagggaat
301 cccagacaca tagcaaatct ggttgcatga tagcattgtg gaccacaaga attctgggga
361 gaccattggt gttaccatcc caactgctcg ttcttaagca aatctcaaca gtggagtttt
421 gtgtattaat atttcagtat acttcacaag gggcataacc ccttttagat cagtaacttc
481 aataccattt cactttaaaa tgagtggcag ccagcctttt gcaccatcac acgcccggca
541 ggactctgtc acactgtgtg acta
//