LOCUS BG068797 728 bp mRNA linear EST 12-MAY-2010
DEFINITION H3069D08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3069D08 3', mRNA sequence.
ACCESSION BG068797
VERSION BG068797.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 728)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG068797.1.
Other_ESTs: H3069D08-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3069 row: D column: 08
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..728
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3069D08-3"
/db_xref="taxon:10090"
/clone="H3069D08"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 192 a 176 c 168 g 192 t
ORIGIN
1 ctttttttct cccttctttt caatttaatc tttgagcccc cacagggttc tttatgttag
61 cttcatgctc agtaaaatgg ccaagtttat ataataagtt ctagatgacc aggatagcta
121 ctgcccctca aaaccagaaa gccaggcagg agaggcaacc tgaagccagc tgggtggttt
181 aactccagaa ctttccaggc aaatacagga aagctgctag tcagcctagg ctacataata
241 taacctgtat caaaggtact tgctgccaag gctgacagcc tgagttcaat cccggggacc
301 tacactaaag atcatggact ctaagttgtc tctgacctct gcacatgtgt gtataatgac
361 aatctgccac ctgaagttac tgagggggtt tgtcaaccct cctactcccc aggccatctt
421 aaatggccta tacactttta tttgggatat tcaaagccaa ggtataggaa ggagatcaag
481 tgaaagggaa agaatgggaa gtggcccaag gcaacagttc caagatggcc aaccttgaag
541 atgcaggcat gtccgctggc tgtgtagcag gtctccccac agattgtgct gtcagcgata
601 cgatgtcttg caacaattca tggaggttgg ggggagattt gctgcctgcc tctctcccat
661 ctcccattca tattagtctc ctcacaaggc ttggtttggg tggcccatga aatgccagat
721 gccatgtc
//