LOCUS BG068744 658 bp mRNA linear EST 12-MAY-2010
DEFINITION H3068H03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3068H03 3', mRNA sequence.
ACCESSION BG068744
VERSION BG068744.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 658)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG068744.1.
Other_ESTs: H3068H03-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3068 row: H column: 03
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..658
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3068H03-3"
/db_xref="taxon:10090"
/clone="H3068H03"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 195 a 168 c 121 g 174 t
ORIGIN
1 gctctctcct tgtttgtttt atcccattaa ttaatttatt tattcacttt acatcttgat
61 cacagccctg ctttactccc agtccctgtc tcacacaatt cctcccccat cctccccttc
121 tactctaaga aggggcaaat ccctcccccc atctgggtag tagcctggta tatcaagtta
181 ctgcagaact aggcagatcc tctcatagct gcttctcttt gggcaccagg cccagaaaaa
241 actgcctttc atgcccacag gaccaaagcc cgctgtgctt cctggtatga tctacaaagt
301 gttttagtta ttaaaaacag agcatgggag gaacaggcta ctccttctgg caagagctca
361 aggccagttg cttcacttgt atattgactg acctcagcag catctctttc aggaggaatt
421 gttaaaggcc attgaattga aacctctcac cctataaaag taaaattgag tcacagggaa
481 aatcctcaga ggaagcatcg gttgtcaaag tacacagaaa gcttgaaagt aaccagcaac
541 taagcactga gaggaaagca tcaatggaaa cctagaaaca acagggacag tccaaataga
601 agttagtagt cctcatagtc acagtaacag caagtgaatg cactcctctt tatatata
//