LOCUS       BG068522                 549 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3066E02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3066E02 3', mRNA sequence.
ACCESSION   BG068522
VERSION     BG068522.2
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG068522.1.
            Other_ESTs: H3066E02-5
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3066  row: E  column: 02
            Seq primer: -21M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3066E02-3"
                     /db_xref="taxon:10090"
                     /clone="H3066E02"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          137 a          114 c          113 g          185 t
ORIGIN      
        1 gtaacttagg cttcttgatg cctttaagaa cattgctgac cttacatttt atatgggact
       61 attgttttgt atttgtggac ctttaaaaga attatctaaa aacaacaaaa atctccagcc
      121 tttaaagaaa ctagttttgc ctctgtccgt cttcatctct ctcatcctcc aatgctgttg
      181 ttattggata ttacataatt cagttgttgg aacccctaac atttccagag aagtgttatg
      241 ccatattaaa tgtcatttga tggggtttta tgaagtggca tcccagacgt tcatgtctat
      301 tgcttcttgt ttttccttgg caggtatggg ttcatagaag gaaatgtggt aattccgagg
      361 attcatccca acgctatctg tgcagccaac cacacaggag tatatatcct cgtcacgtcc
      421 aacacctccc actatgacac atattgcttc aatgcctcag gttggttcca agtgcatggt
      481 tttggggttg cctgtgggag cattggctga caacagttct gttgaagcat gggtggagct
      541 ttctgaagc
//