LOCUS BG068390 562 bp mRNA linear EST 12-MAY-2010
DEFINITION H3064H11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3064H11 3', mRNA sequence.
ACCESSION BG068390
VERSION BG068390.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 562)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG068390.1.
Other_ESTs: H3064H11-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3064 row: H column: 11
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..562
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3064H11-3"
/db_xref="taxon:10090"
/clone="H3064H11"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 137 a 124 c 175 g 126 t
ORIGIN
1 attactaaag ggatttatta gagtgactta caaggcagag cctgggtagg ccacgtgtag
61 ccatctgcaa ccagagaggc tgggaaccca ggaggctggg tgcctcagca ggcccagtct
121 ggtgccacaa gcttggagaa ctttctggag agctgctggt ctttagctca cattggaagg
181 ccaaagaagc tgggctctga tgatagcaaa caatgctggt ggtggtggcg gcggcagcag
241 cagcagcagg tagatgttct cgcccactta gtccggtgtg gtggaggcat tttctcaatt
301 gaggttccct cttcccagat gactctagct tctgtcaagt tgccaaacag cacaggagcc
361 tagtaggaga ttaaaagtgt tctccaggga tgagccggaa gggacctttg tttcttggaa
421 accctcacac agtgtctcca tggcacccat caccagcaag gagagggatg gaagggtggg
481 acctagtcat ctttgccaag gagggggaag tcaaggattg ggagtatggg ctgagcattg
541 ctgactttta ggagcatcaa gc
//