LOCUS BG067893 810 bp mRNA linear EST 12-MAY-2010
DEFINITION H3059D07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3059D07 3', mRNA sequence.
ACCESSION BG067893
VERSION BG067893.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 810)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG067893.1.
Other_ESTs: H3059D07-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3059 row: D column: 07
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..810
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3059D07-3"
/db_xref="taxon:10090"
/clone="H3059D07"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 161 a 224 c 220 g 205 t
ORIGIN
1 attgcacatg gtccttttta ttttctgcag agaaaaggct tccttcctct gcagacctaa
61 agcctcaggc tggcagagtg acctcatcta gatgtgatca tgacgttaaa aagcattcct
121 gggtagtgat tttagacaga tggtgctgac atgaatgggc tcagagcagt aatggagggg
181 ggcaatgaat cacaggacag ctccaggcgt gttcccatta ccgctggatc aggacggaca
241 gggctgctga gaggtcccat gactccaatg tcttcgtggc gggaggagag ctcctggctg
301 aattcccgcc ttcggacatg tctggttgat ggcgagttgc tttcccgaga ggcttccctc
361 ccatcagttc ttcacatctc ccagacggag ctgggcaaag gaggtagcca gctgcagtgc
421 ttcctgaagg cagcccacgt tcttgcctgt ggcctgggcg gacatgtcct tgccaccgcc
481 tttaccatcc atcaggcctg aaacctgctg gacccactcg ctggctttca gtccccggtt
541 ggctgcattc tggggaacct ggcacaagca cgtgatcttg ccagcctcat tgtccaccgt
601 gaacagcatg gcagatgtct gaggagaatg cgtcttgaaa agcttcaacg cttcattcag
661 ggccttggcc gatgctccac tctccatctc caggatgacc agcggctggt tggggttgct
721 gtcaatcagc tgctttgtct tctctaacac tcgcttctgc acatcggctt tgctggcccg
781 gtctaggtca tccatgactt tcttcaggga
//