LOCUS BG067289 938 bp mRNA linear EST 26-JAN-2001
DEFINITION H3052F10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3052F10 3', mRNA sequence.
ACCESSION BG067289
VERSION BG067289.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 938)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Other_ESTs: H3052F10-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3052 row: F column: 10
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..938
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3052F10-3"
/db_xref="taxon:10090"
/clone="H3052F10"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 244 a 204 c 226 g 264 t
ORIGIN
1 ggtttctgct gcaattaaca ggtcatttac atactgcaca agagttatat ctgggttgtt
61 gactctttac tcacccaggt cctcatgcaa agcttcacca aaaatagtgg gtgaattctt
121 gtagccttaa ggcaggtaag gccaggtgag ttgttcttta atgcctcttt caggatcatg
181 accctcaaag gcaaagtatt tagctaaggg taagctgaat aaagcattct ttaggtccag
241 aacagaacac catttccatc tggaggtaga gaatcaatag ggtataagga ttaggtacca
301 ttggtgtatg tccattaccc tgccattaac ttcccatagg tcctggacag ggcagttgcc
361 attggacttt ggcttcttca caggcaataa tgggatgttc caggctgact ggcatggcct
421 aaggattcct aaggccaaca gtttccagat acagggaacc atccctttct gtgcatcttg
481 gaacatggga cattggagga cctgtactgg gtcagctcct agcttgagct ctacaaatac
541 agggtgtcaa tattttgcca gccccatgcc tccagactca tttgtttgaa aaagcctatg
601 ggatccaggg agtggaatgg tatggtttgt atatacttgg ccaagggact ggcactatta
661 gaaggtgtgg ccctgttgga gtaggtgtgt cactgtgagt gtgggcttta agacctcatc
721 ctagctgact gggagccagt attctagaag cctcagatga agatgtagac tctcagctct
781 gcatgtacat gcctgccaga catgccatag tcctgcttga tgatatagtc taaactcttg
841 agctgtacca gcctaattaa atgtggtctt atagagttgc tggtcatgtg ctgctacaca
901 gaaaacctac tgagacacag tcactccttc agcacata
//