LOCUS BG066494 837 bp mRNA linear EST 12-MAY-2010
DEFINITION H3043F04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3043F04 3', mRNA sequence.
ACCESSION BG066494
VERSION BG066494.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 837)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG066494.1.
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3043 row: F column: 04
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..837
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3043F04-3"
/db_xref="taxon:10090"
/clone="H3043F04"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 230 a 199 c 188 g 220 t
ORIGIN
1 cgttttttcg agacagggtt tctggaactc actctgtaga ccaagctggc ctcaaactca
61 gaaatccgcc tgcctctgca tcccaagtgc tgggattaaa ggtgtaagac accaccgccc
121 ggcaacagtc attgaattga gagtgaacct ctcgccccat acaggcatgt gtgtccccat
181 ggccaaaact tggctatttt tgctattcta aggccctttc acatatgtgc tcgttttaga
241 gccacatagg atcagggaaa gagctgcccc ccatctccag aggacggctg ggttccacca
301 accctcccct gtcctctatt ccatttcact gttagattgt caaggttggc ctgagtccta
361 aacagacatt ttacagcagt agcaagggaa acagccatga gttagtatga ctgttgagca
421 cccactgatt ggtctggtca gaatggtcag gggaatgctg ggggaataat taagggagag
481 cagaaacagg gctagggagc acggagatct ctcaggtaca gggcagaacc atctataact
541 ggacactact ttttattctt aaacattttg aaagcttttc tttaatgaaa tttttactga
601 gttttcaagt gttctggtgg ttaaaacaaa acaaatattg acaaaggcta tggttcaaaa
661 actaaaaact caaaatgaca tgattctact tataacccta gtgtttcctg gaggcctggg
721 aaatggcagc catgagaagt gccaggtgct cctgcctcta ccactgcact cctgcagact
781 acctttcacc ataacgactt cgatgctgag agaaatgctg tgactagcag ccatttt
//