LOCUS BG066436 768 bp mRNA linear EST 12-MAY-2010
DEFINITION H3043A03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3043A03 3', mRNA sequence.
ACCESSION BG066436
VERSION BG066436.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 768)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG066436.1.
Other_ESTs: H3043A03-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3043 row: A column: 03
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..768
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3043A03-3"
/db_xref="taxon:10090"
/clone="H3043A03"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 202 a 198 c 183 g 185 t
ORIGIN
1 atgactcaac tactaaatcc acaagcaacg gttcagacac ggtgcttctg aagtgttctc
61 acccctccca aacaggcgca actgcatcga ggagagggtg gacttcccca cctctgctca
121 ggcatcagaa ggtagatggg ggtcattact gctgagcgcc acagcagctg gaacaggtat
181 cccaagtgag ttggcagcgg caatcactgg tgagcctgcc agaggtccaa ggggtgaagg
241 agttggcact gaagaaatcc ctgacatcat attggcgctg ctgacaggtg tgctgccccc
301 tgccatgcta gtggaactca ttcgagcctg gtccttcaca acagtgtaga agtaagggtg
361 ctccatggcc tctcttgcag tgagccgtga ctggtggtca tatcgaagca gcttgtccag
421 aaaatccaag gcctcagggc tgacaagatg ctggttttca ctgtggacaa agcgttccca
481 tcgcttacgg gagtgtctgc ccaagatatc gttgaaacgt ggatctaatt caatgttgta
541 cttgtcaata tagtcatata aatcttctgt tcccagaacc ttggctatcc tcaccaactg
601 atcataattg tcatgtccat ggaaaaatgg ctccttccgg aagatcatac tggccagcat
661 acaacccaag ctccacatat ccaaactata atcgtacatc tgataatcta caagtagctc
721 tggacctttg aaatatcgag aagcactcgg acattatact cttggcct
//