LOCUS BG064500 685 bp mRNA linear EST 12-MAY-2010
DEFINITION H3020E01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3020E01 3', mRNA sequence.
ACCESSION BG064500
VERSION BG064500.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 685)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG064500.1.
Other_ESTs: H3020E01-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3020 row: E column: 01
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..685
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3020E01-3"
/db_xref="taxon:10090"
/clone="H3020E01"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 220 a 168 c 132 g 165 t
ORIGIN
1 agaaatgaca ccacagggtg gactttattt taaagctcac gaggtgttca caatgatcac
61 atgatccaca cgtgtgtctc ccgtgtcaca cctccacggg acaatgcact gtgtggtgat
121 agttcacagc cctgcttcgc atgctgctac ggttcactag ctctgttgta ctcttgactt
181 ggtaataatg aaagcaaaat cactctactg gacagactta atttttgaaa gcccttatgc
241 aaatcagact tagtctcatt tataaacaac cacagccaca cacatgcgga aatgaagagc
301 aaaatgcaaa acaaaacaaa acccttggca agaataactg cttaagattc tgatcccagc
361 gtgtgaccaa gcagtgtagt tcatatcctc agaagacgac cccacctcct cagtgtatga
421 gcagcaatac tgtacagagc tcagtgtggg gcacaactcc acaggaccct gacaccaaag
481 tcattctcac ttagggtcag agactacaca gattcaagct tttccttttt tcccctcata
541 atacacaaaa tttctagaca gtcttaaaaa aaagaaaata taaatagact cagtctgtta
601 tacaggatca catactatgg caaacacatc tcatgaagtg ggggagggag ccagcacaga
661 gcctagtcca ggtccatgtt gacag
//