LOCUS       BG063668                 542 bp    mRNA    linear   EST 26-JAN-2001
DEFINITION  H3010A11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3010A11 3', mRNA sequence.
VERSION     BG063668.1
DBLINK      BioSample: SAMN00163148
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
            Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
  TITLE     Verification and initial annotation of NIA mouse 15K cDNA clone set
  JOURNAL   Unpublished
COMMENT     Other_ESTs: H3010A11-5
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            This clone set has been freely distributed to the community. Please
            visit for details.
            Plate: H3010  row: A  column: 11
            Seq primer: -21M13 Forward
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Mus musculus"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          121 a          146 c           96 g          179 t
        1 cttctaatct ctctccaggt attcctacct aaccttaact tttcctcggg ttcaagaccc
       61 ttggaaaggc ctgtatactt attttgtgta ccatacttcc tctttgttcc tactctttct
      121 tcccgcttta cttctgatag actgtcctga atttcctcta gaattttcag ccctgcctta
      181 agcactatat aacatgtgaa aaggaacaaa agggctccta acactagaaa aagttcaagg
      241 ccaaacatac ctggtaaagc catttctcac ttccgttccc cagctgatga gttctgaatt
      301 cggcagttga atccttctca acagtctgct ttacgggaac ctttatcacc gtcgttcccc
      361 agctgatgag ttctgaattc ggcagttgaa tccttctcaa cagtctgtgt tacgggaact
      421 tataaccttg attcgcagtt ctggttctgg aatgaagtat ccctcctgcg ccagtccgga
      481 gttttttctc gtcccggatt ttctcgtccc ggaattcggc accaattgtt attcgacgcg
      541 tt