LOCUS       BG063401                 581 bp    mRNA    linear   EST 26-JAN-2001
DEFINITION  H3006G12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3006G12 3', mRNA sequence.
ACCESSION   BG063401
VERSION     BG063401.1
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
            Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
  TITLE     Verification and initial annotation of NIA mouse 15K cDNA clone set
  JOURNAL   Unpublished
COMMENT     Other_ESTs: H3006G12-5
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3006  row: G  column: 12
            Seq primer: -21M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3006G12-3"
                     /db_xref="taxon:10090"
                     /clone="H3006G12"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          173 a          136 c          129 g          143 t
ORIGIN      
        1 aaatctactt attttattta tgtgagtaca ctgtagttgt cttcagacac accagagagg
       61 gcagcagatc ttattacaga tggctgtgaa gccgggcgcg gtggtgcacg cctttaatcc
      121 cagcactcgg gaggcagagg caggtggatt tctgagttcg aggccagcct ggtctacaaa
      181 gtgagttcca ggagagccag ggctatacag agaaacctgt ctcgaaaaac caaaaaagaa
      241 aaaaaaaaag atggctgtga gccaccatgt agttgctggg tttgaaccca ggacctttgg
      301 aagagcaatc agtgctctta accactgagc catctctccc tcttcatgag gcttaaccag
      361 agagcaaagt ctctgctggg cttttatgag acttcctttg tcaatgcaac taatctgaat
      421 ctcttcacgt cagccttagg cagactcttc agacaagggc aaaaagtagc cacattcttg
      481 aataccacaa aagcagtctc taggccacat attgaaattc tccactgaaa tctcttggac
      541 caggtccaaa taattcaaat cactctcagt aacaaagtct t
//