LOCUS BG063025 744 bp mRNA linear EST 12-MAY-2010
DEFINITION H3001B11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3001B11 3', mRNA sequence.
ACCESSION BG063025
VERSION BG063025.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 744)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG063025.1.
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3001 row: B column: 11
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..744
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3001B11-3"
/db_xref="taxon:10090"
/clone="H3001B11"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 223 a 161 c 154 g 206 t
ORIGIN
1 ccttttaaag ttttttattt tttatttttt ttgcattatt ttaaaaaatt ccatcaatac
61 cctggaagcc tttagaacag ttttatcttg aaacacagga cacttttctc ccagtgcaca
121 gaattcaagt ttacgtggtt cagcttaaga agtgtatgtt tcatgtttaa gaggacaaca
181 gactcaactt atcactataa gagaacagct gtcccagcat caatgggata acacaccact
241 aacaacttcc tgtacaacag caagatggtc aatccctgtt tgttatccac agggacagca
301 tgacaaggag agtccctatc tgacttaaag caggttagcc ccttctaatc tctgctgcta
361 ggagtcaaga cttgtaactt atgaggttta agcttccaat gacccaggaa cccaggccct
421 ggctgtgact actaatcata tatttatagg gctacctctg ctcagatgca cattcccaca
481 gcttctctta gagcaggaga gcccagaggc tatgtgggac actggcaaag gctggagtta
541 agacccatga aaggcagcag ggagcttact gtaagcatga tgacaaaatg aacatgggtt
601 aatactgagg taaatgaaca ctaaatccat atggagcctg taaacgtcct atactttctt
661 ctgtcttcaa ggacagaaaa tggggctaga ggagaggcct gccagacctt atggggacac
721 agcttttgga gtaccttcat taag
//