LOCUS       BE470633                 287 bp    mRNA    linear   EST 28-JUL-2000
DEFINITION  WHE0262_C02_C02ZS Wheat drought-stressed seedling cDNA library
            Triticum aestivum cDNA clone WHE0262_C02_C02, mRNA sequence.
ACCESSION   BE470633
VERSION     BE470633.1
DBLINK      BioSample: SAMN00160041
KEYWORDS    EST.
SOURCE      Triticum aestivum (bread wheat)
  ORGANISM  Triticum aestivum
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP
            clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Anderson,O.D., Chao,S., Choi,D.W., Close,T.J., Fenton,R.D.,
            Han,P.S., Hsia,C.C., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J.,
            Seaton,C.L. and Tong,J.C.
  TITLE     The structure and function of the expressed portion of the wheat
            genomes - Drought-stressed seedling cDNA library
  JOURNAL   Unpublished
COMMENT     Contact: Olin Anderson
            US Department of Agriculture, Agriculture Research Service, Pacific
            West Area, Western Regional Research Center
            800 Buchanan Street, Albany, CA 94710, USA
            Tel: 5105595773
            Fax: 5105595818
            Email: oandersn@pw.usda.gov
            Sequence have been trimmed to remove vector sequence and low
            quality sequence with phred score less than 20
            Seq primer: Stratagene SK primer.
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Triticum aestivum"
                     /mol_type="mRNA"
                     /cultivar="Chinese Spring"
                     /db_xref="taxon:4565"
                     /clone="WHE0262_C02_C02"
                     /tissue_type="Seedling without endosperm"
                     /clone_lib="SAMN00160041 Wheat drought-stressed seedling
                     cDNA library"
                     /dev_stage="Five day old seedling"
                     /lab_host="E. coli SOLR"
                     /note="Vector: Lambda Uni-ZAP XR, excised phagemid;
                     Site_1: EcoRI; Site_2: XhoI; Seeds were
                     surface-sterilized, germinated and grown aseptically in
                     the dark at room temperature on filter paper with water,
                     nystatin and cefotaxime in covered crystallization dishes.
                     Five-day old seedlings were incubated for one day at 90%
                     RH. After removing endosperm, seedlings were transferred
                     to desiccator jar containing saturated MgSO4 at room
                     temperature for 24 hr. The tissue, total RNA, and poly(A)
                     RNA were prepared, a cDNA library was made, and the cDNA
                     clones were in vivo excised to give pBluescript phagemids
                     in the TJ Close lab (Choi, Close, Fenton) at the
                     University of California, Riverside. Plasmid DNA
                     preparations and DNA sequencing were performed in the OD
                     Anderson lab (all other authors)."
BASE COUNT           96 a           62 c           77 g           52 t
ORIGIN      
        1 gcgccgccgg ccaggaagcc catttaccag ccatgtttgg acgcatgcca aggaagacca
       61 gcaacaacac caagtactac gaggtgcttg gtgtatccaa gacagcaacc ccggatgagt
      121 tgaagaaagc ttaccggaaa gctgctataa aaaaccatcc tgataagggc ggagaccctg
      181 agaagtttaa agaattggca caagcttatg atgttctcaa tgaccctgaa aagagggaga
      241 tttatgacca atatggagaa gatgcattca aagaagggat gggaggc
//