LOCUS       BE470556                 603 bp    mRNA    linear   EST 28-JUL-2000
DEFINITION  WHE0261_C03_C03ZS Wheat drought-stressed seedling cDNA library
            Triticum aestivum cDNA clone WHE0261_C03_C03, mRNA sequence.
ACCESSION   BE470556
VERSION     BE470556.1
DBLINK      BioSample: SAMN00160041
KEYWORDS    EST.
SOURCE      Triticum aestivum (bread wheat)
  ORGANISM  Triticum aestivum
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP
            clade; Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Anderson,O.D., Chao,S., Choi,D.W., Close,T.J., Fenton,R.D.,
            Han,P.S., Hsia,C.C., Kang,Y., Lazo,G.R., Miller,R., Rausch,C.J.,
            Seaton,C.L. and Tong,J.C.
  TITLE     The structure and function of the expressed portion of the wheat
            genomes - Drought-stressed seedling cDNA library
  JOURNAL   Unpublished
COMMENT     Contact: Olin Anderson
            US Department of Agriculture, Agriculture Research Service, Pacific
            West Area, Western Regional Research Center
            800 Buchanan Street, Albany, CA 94710, USA
            Tel: 5105595773
            Fax: 5105595818
            Email: oandersn@pw.usda.gov
            Sequence have been trimmed to remove vector sequence and low
            quality sequence with phred score less than 20
            Seq primer: Stratagene SK primer.
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Triticum aestivum"
                     /mol_type="mRNA"
                     /cultivar="Chinese Spring"
                     /db_xref="taxon:4565"
                     /clone="WHE0261_C03_C03"
                     /tissue_type="Seedling without endosperm"
                     /clone_lib="SAMN00160041 Wheat drought-stressed seedling
                     cDNA library"
                     /dev_stage="Five day old seedling"
                     /lab_host="E. coli SOLR"
                     /note="Vector: Lambda Uni-ZAP XR, excised phagemid;
                     Site_1: EcoRI; Site_2: XhoI; Seeds were
                     surface-sterilized, germinated and grown aseptically in
                     the dark at room temperature on filter paper with water,
                     nystatin and cefotaxime in covered crystallization dishes.
                     Five-day old seedlings were incubated for one day at 90%
                     RH. After removing endosperm, seedlings were transferred
                     to desiccator jar containing saturated MgSO4 at room
                     temperature for 24 hr. The tissue, total RNA, and poly(A)
                     RNA were prepared, a cDNA library was made, and the cDNA
                     clones were in vivo excised to give pBluescript phagemids
                     in the TJ Close lab (Choi, Close, Fenton) at the
                     University of California, Riverside. Plasmid DNA
                     preparations and DNA sequencing were performed in the OD
                     Anderson lab (all other authors)."
BASE COUNT          162 a          153 c          160 g          128 t
ORIGIN      
        1 gccgggatca gatccatctc tccgcgcgcg atgtctgagg tggcagcgac ggcggggcag
       61 gcgtacatga gacagctctc caagcacccg ctccgcacca aggccatcac ctccggggtg
      121 ctggccggct gcagcgacgc cgtcgcccag aagatctccg gcgtcaagaa gctccagctc
      181 aggaggctcc tcctcataat gctctatgga tttgcatacg caggaccatt cggtcatttt
      241 ttccacaagc ttatggacaa gattttcaag ggacagaaga aaggaaaaga aactacagcc
      301 aagaaggtca tagtggagca gctaactgta tcaccatgga acaatatgat gttcatgatg
      361 tactacggtc tggtagttga agggaggcca ttcgggcaag taaagagcaa ggtcaagaag
      421 gactttgcaa acatccaatt gacagcttgg aagttctggc caatagttag ctggatcaat
      481 tatgagtaca tgccactcca gctccgagtt cttttcgcca gctccgttgc atcatgctgg
      541 gcagtgtttc tgaacctgaa agcagcaaga tccattgccg gcactagtaa gaatgcataa
      601 aag
//