LOCUS       BC070280                 967 bp    mRNA    linear   HUM 15-JUL-2006
DEFINITION  Homo sapiens dihydrofolate reductase, mRNA (cDNA clone MGC:88273
            IMAGE:6714220), complete cds.
ACCESSION   BC070280
VERSION     BC070280.1
KEYWORDS    MGC.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
            Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
            Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
            Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
            Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
            Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
            Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
            Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
            Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
            McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
            Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
            Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
            Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
            Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
            Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
            Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
            Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
            Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
  CONSRTM   Mammalian Gene Collection Program Team
  TITLE     Generation and initial analysis of more than 15,000 full-length
            human and mouse cDNA sequences
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
   PUBMED   12477932
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NIH MGC Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAY-2004) National Institutes of Health, Mammalian
            Gene Collection (MGC), Bethesda, MD 20892-2590, USA
  REMARK    NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT     Contact: MGC help desk
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: NCI
            cDNA Library Preparation: Michael Brownstein /  Ted Usdin
            Laboratory
            cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
            DNA Sequencing by: Sequencing Group at the Stanford Human Genome
            Center, Stanford University School of Medicine, Stanford, CA  94305
            Web site:       http://www-shgc.stanford.edu
            Contact:  (Dickson, Mark) mcd@paxil.stanford.edu
            Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
            R. M.
            
            Clone distribution: MGC clone distribution information can be found
            through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
            Series: IRAL Plate: 57 Row: m Column: 9
            This clone was selected for full length sequencing because it
            passed the following selection criteria: matched mRNA gi: 31341213.
FEATURES             Location/Qualifiers
     source          1..967
                     /db_xref="H-InvDB:HIT000264238"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="MGC:88273 IMAGE:6714220"
                     /tissue_type="Pooled, 40 cell lines"
                     /clone_lib="NIH_MGC_127"
                     /lab_host="DH10B"
                     /note="Vector: pDNR-LIB"
     gene            1..967
                     /gene="DHFR"
                     /db_xref="GeneID:1719"
                     /db_xref="HGNC:HGNC:2861"
                     /db_xref="MIM:126060"
     CDS             38..601
                     /gene="DHFR"
                     /codon_start=1
                     /product="dihydrofolate reductase"
                     /protein_id="AAH70280.1"
                     /db_xref="GeneID:1719"
                     /db_xref="HGNC:HGNC:2861"
                     /db_xref="MIM:126060"
                     /translation="MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSV
                     EGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTE
                     QPELANKVDMLWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLL
                     PEYPGVLSDVQEEKGIKYKFEVYEKND"
BASE COUNT          302 a          201 c          189 g          275 t
ORIGIN      
        1 tgtaacgagc gggctcggag gtcctcccgc tgctgtcatg gttggttcgc taaactgcat
       61 cgtcgctgtg tcccagaaca tgggcatcgg caagaacggg gacctgccct ggccaccgct
      121 caggaatgaa ttcagatatt tccagagaat gaccacaacc tcttcagtag aaggtaaaca
      181 gaatctggtg attatgggta agaagacctg gttctccatt cctgagaaga atcgaccttt
      241 aaagggtaga attaatttag ttctcagcag agaactcaag gaacctccac aaggagctca
      301 ttttctttcc agaagtctag atgatgcctt aaaacttact gaacaaccag aattagcaaa
      361 taaagtagac atgctctgga tagttggtgg cagttctgtt tataaggaag ccatgaatca
      421 cccaggccat cttaaactat ttgtgacaag gatcatgcaa gactttgaaa gtgacacgtt
      481 ttttccagaa attgatttgg agaaatataa acttctgcca gaatacccag gtgttctctc
      541 tgatgtccag gaggagaaag gcattaagta caaatttgaa gtatatgaga agaatgatta
      601 atatgaaggt gttttctagt ttaagttgtt ccccctccct ctgaaaaaag tatgtatttt
      661 tacattagaa aaggtttttt gttgacttta gatctataat tatttctaag caacttgttt
      721 ttattcccca ctactcttgt ctctatcaga taccatttat gagacattct tgctataact
      781 aagtgcttct ccaagacccc aactgagtcc ccagcacctg ctacagtgag ctgccattcc
      841 acacccatca catgtggcac tcttgccagt ccttgacatt gtcgggcttt tcacatgttg
      901 gtaatattta ttaaagatga agatccacat acccttcaaa aaaaaaaaaa aaaaaaaaaa
      961 aaaaaaa
//