LOCUS       BC070123                 641 bp    mRNA    linear   HUM 15-JUL-2006
DEFINITION  Homo sapiens interleukin 4, mRNA (cDNA clone MGC:88101
            IMAGE:30415895), complete cds.
ACCESSION   BC070123
VERSION     BC070123.1
KEYWORDS    MGC.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
            Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
            Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
            Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
            Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
            Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
            Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
            Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
            Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
            McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
            Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
            Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
            Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
            Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
            Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
            Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
            Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
            Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
  CONSRTM   Mammalian Gene Collection Program Team
  TITLE     Generation and initial analysis of more than 15,000 full-length
            human and mouse cDNA sequences
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
   PUBMED   12477932
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NIH MGC Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAY-2004) National Institutes of Health, Mammalian
            Gene Collection (MGC), Bethesda, MD 20892-2590, USA
  REMARK    NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT     Contact: MGC help desk
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: Narayan Bhat
            cDNA Library Preparation: CLONTECH Laboratories, Inc.
            cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
            DNA Sequencing by: Sequencing Group at the Stanford Human Genome
            Center, Stanford University School of Medicine, Stanford, CA  94305
            Web site:       http://www-shgc.stanford.edu
            Contact:  (Dickson, Mark) mcd@paxil.stanford.edu
            Dickson, M., Schmutz, J., Grimwood, J., Rodriquez, A., and Myers,
            R. M.
            
            Clone distribution: MGC clone distribution information can be found
            through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
            Series: IRAL Plate: 57 Row: p Column: 16
            This clone was selected for full length sequencing because it
            passed the following selection criteria: matched mRNA gi: 27477090.
FEATURES             Location/Qualifiers
     source          1..641
                     /db_xref="H-InvDB:HIT000264081"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="MGC:88101 IMAGE:30415895"
                     /tissue_type="Blood, peripheral blood mononuclear cells
                     (PBMC) cytoplasmic poly A RNA is pooled sample from 3 and
                     6 hr after stimulation with PMA and Ionomycin. These
                     reagents are used to activate T- cells that results in
                     lymphokine and cytokine production."
                     /clone_lib="NIH_MGC_191"
                     /lab_host="DH10B"
                     /note="Vector: pDNR-LIB"
     gene            1..641
                     /gene="IL4"
                     /gene_synonym="BSF1"
                     /gene_synonym="IL-4"
                     /db_xref="GeneID:3565"
                     /db_xref="HGNC:HGNC:6014"
                     /db_xref="MIM:147780"
     CDS             63..524
                     /gene="IL4"
                     /gene_synonym="BSF1"
                     /gene_synonym="IL-4"
                     /codon_start=1
                     /product="interleukin 4"
                     /protein_id="AAH70123.1"
                     /db_xref="GeneID:3565"
                     /db_xref="HGNC:HGNC:6014"
                     /db_xref="MIM:147780"
                     /translation="MGLTSQLLPPLFFLLACAGNFVHGHKCDITLQEIIKTLNSLTEQ
                     KTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQ
                     LIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS"
BASE COUNT          202 a          149 c          129 g          161 t
ORIGIN      
        1 atcgttagct tctcctgata aactaattgt ctcacattgt cactgcaaat cgacacctat
       61 taatgggtct cacctcccaa ctgcttcccc ctctgttctt cctgctggca tgtgccggca
      121 actttgtcca cggacacaag tgcgatatca ccttacagga gatcatcaaa actttgaaca
      181 gcctcacaga gcagaagact ctgtgcaccg agttgaccgt aacagacatc tttgctgcct
      241 ccaagaacac aactgagaag gaaaccttct gcagggctgc gactgtgctc cggcagttct
      301 acagccacca tgagaaggac actcgctgcc tgggtgcgac tgcacagcag ttccacaggc
      361 acaagcagct gatccgattc ctgaaacggc tcgacaggaa cctctggggc ctggcgggct
      421 tgaattcctg tcctgtgaag gaagccaacc agagtacgtt ggaaaacttc ttggaaaggc
      481 taaagacgat catgagagag aaatattcaa agtgttcgag ctgaatattt taatttatga
      541 gtttttgata gctttatttt ttaagtattt atatatttat aactcatcat aaaataaagt
      601 atatatagaa tcaaaaaaaa aaaaaaaaaa aaaaaaaaaa a
//