LOCUS BC054891 1030 bp mRNA linear HUM 05-NOV-2003
DEFINITION Homo sapiens RAS p21 protein activator (GTPase activating protein)
1, mRNA (cDNA clone IMAGE:6663865), partial cds.
ACCESSION BC054891
VERSION BC054891.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1030)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1030)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (03-JUL-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: CLONTECH
cDNA Library Preparation: CLONTECH Laboratories, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 48 Row: l Column: 3
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 12545405.
FEATURES Location/Qualifiers
source 1..1030
/db_xref="H-InvDB:HIT000259481"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="IMAGE:6663865"
/tissue_type="Placenta"
/clone_lib="NIH_MGC_79"
/lab_host="DH10B"
/note="Vector: pDNR-LIB"
gene 1..>1030
/gene="RASA1"
/gene_synonym="GAP"
/gene_synonym="p120GAP"
/gene_synonym="RASA"
/gene_synonym="RASGAP"
/db_xref="GeneID:5921"
/db_xref="MIM:139150"
CDS 91..>1030
/gene="RASA1"
/gene_synonym="GAP"
/gene_synonym="p120GAP"
/gene_synonym="RASA"
/gene_synonym="RASGAP"
/codon_start=1
/product="RASA1 protein"
/protein_id="AAH54891.1"
/db_xref="GeneID:5921"
/db_xref="MIM:139150"
/translation="MKGWYHGKLDRTIAEERLRQAGKSGSYLIRESDRRPGSFVLSFL
SQMNVVNHFRIIAMCGDYYIGGRRFSSLSDLIGYYSHVSCLLKGEKLLYPVAPPEPVE
DRRRVRAILPYTKVPDTDEISFLKGDMFIVHNELEDGWMWVTNLRTDEQGLIVEDLVE
EVGREEDPHEGKIWFHGKISKQEAYNLLMTVGQVCSFLVRPSDNTPGDYSLYFRTNEN
IQRFKICPTPNNQFMMGGRYYNSIGDIIDHYRKEQIVEGYYLKEPVPMQDQEQVLNDT
VDGKEIYNTIRRKTKDAFYKNIVKKGYLLKKKKKKKK"
misc_feature 100..327
/gene="RASA1"
/gene_synonym="GAP"
/gene_synonym="p120GAP"
/gene_synonym="RASA"
/gene_synonym="RASGAP"
/note="SH2; Region: SH2 domain"
/db_xref="CDD:pfam00017"
misc_feature 418..576
/gene="RASA1"
/gene_synonym="GAP"
/gene_synonym="p120GAP"
/gene_synonym="RASA"
/gene_synonym="RASGAP"
/note="SH3; Region: SH3 domain. SH3 (Src homology 3)
domains are often indicative of a protein involved in
signal transduction related to cytoskeletal organisation.
First described in the Src cytoplasmic tyrosine kinase.
The structure is a partly opened beta barrel"
/db_xref="CDD:pfam00018"
misc_feature 610..837
/gene="RASA1"
/gene_synonym="GAP"
/gene_synonym="p120GAP"
/gene_synonym="RASA"
/gene_synonym="RASGAP"
/note="SH2; Region: SH2 domain"
/db_xref="CDD:pfam00017"
BASE COUNT 357 a 169 c 233 g 271 t
ORIGIN
1 ggggataccg ttgaccgctc ctccaactaa ccagtaagtt aagactgctg ttcaggaatt
61 tgggaagctg gctccagaaa agaagtggaa atgaaggggt ggtatcacgg aaaacttgac
121 agaacgatag cagaagaacg cctcaggcag gcagggaagt ctggcagtta tcttataaga
181 gagagtgatc ggaggccagg gtcctttgta ctttcatttc ttagccagat gaatgttgtc
241 aaccatttta ggattattgc tatgtgtgga gattactaca ttggtggaag acgtttttct
301 tcactgtcag acctaatagg ttattacagt catgtttctt gtttgcttaa aggagaaaaa
361 ttactttacc cagttgcacc accagagcca gtagaagata gaaggcgtgt acgagctatt
421 ctaccttaca caaaagtacc agacactgat gaaataagtt tcttaaaagg agatatgttc
481 attgttcata atgaattaga agatggatgg atgtgggtta caaatttaag aacagatgaa
541 caaggcctta ttgttgaaga cctagtagaa gaggtgggcc gggaagaaga tccacatgaa
601 ggaaaaatat ggttccatgg gaagatttcc aaacaggaag cttataattt actaatgaca
661 gttggtcaag tctgcagttt tcttgtgagg ccctcagata atactcctgg cgattattca
721 ctttatttcc ggaccaatga aaatattcag cgatttaaaa tatgtccaac gccaaacaat
781 cagtttatga tgggaggccg gtattataac agcattgggg acatcataga tcactatcga
841 aaagaacaga ttgttgaagg atattatctt aaggaacctg taccaatgca ggatcaagaa
901 caagtactca atgacacagt ggatggcaag gaaatctata ataccatccg tcgtaaaaca
961 aaggatgcct tttataaaaa cattgttaag aaaggttatc ttctgaaaaa aaaaaaaaaa
1021 aaaaaaaaaa
//