LOCUS BC042146 4384 bp mRNA linear HUM 23-DEC-2003
DEFINITION Homo sapiens KIAA0427, mRNA (cDNA clone MGC:52202 IMAGE:5928756),
complete cds.
ACCESSION BC042146
VERSION BC042146.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4384)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 4384)
AUTHORS Strausberg,R.
TITLE Direct Submission
JOURNAL Submitted (02-JAN-2003) National Institutes of Health, Mammalian
Gene Collection (MGC), Cancer Genomics Office, National Cancer
Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590,
USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 44 Row: p Column: 21
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 7662111.
FEATURES Location/Qualifiers
source 1..4384
/db_xref="H-InvDB:HIT000052660"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:52202 IMAGE:5928756"
/tissue_type="Brain, neuroblastoma"
/clone_lib="NIH_MGC_47"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..4384
/gene="KIAA0427"
/db_xref="GeneID:9811"
CDS 211..2013
/gene="KIAA0427"
/codon_start=1
/product="KIAA0427 protein"
/protein_id="AAH42146.1"
/db_xref="GeneID:9811"
/translation="MENSSAASASSEAGSSRSQEIEELERFIDSYVLEYQVQGLLADK
TEGDGESERTQSHISQWTADCSEPLDSSCSFSRGRAPPQQNGSKDNSLDMLGTDIWAA
NTFDSFSGATWDLQPEKLDFTQFHRKVRHTPKQPLPHIDREGCGKGKLEDGDGINLND
IEKVLPAWQGYHPMPHEVEIAHTKKLFRRRRNDRRRQQRPPGGNKPQQHGDHQPGSAK
HNRDHQKSYQGGSAPHPSGRPTHHGYSQNRRWHHGNMKHPPGDKGEAGAHRNAKETMT
IENPKLEDTAGDTGHSSLEAPRSPDTLAPVASERLPPQQSGGPEVETKRKDSILPERI
GERPKITLLQSSKDRLRRRLKEKVPDEVAVETTTPQQNKMDKLIEILNSMRNNSSDVD
TKLTTFMEEAQNSTNSEEMLGEIVRTIYQKAVSDRSFAFTAAKLCDKMALFMVEGTKF
RSLLLNMLQKDFTVREELQQQDVERWLGFITFLCEVFGTMRSSTGEPFRVLVCPIYTC
LRELLQSQDVKEDAVLCCSMELQSTGRLLEEQLPEMMTELLASARDKMLCPSESMLTR
SLLLEVIELHANSWNPLTPPITQYYNRTIQKLTA"
misc_feature 1303..1959
/gene="KIAA0427"
/note="nic; Region: Large alpha helical domain involved in
protein-protein interactions in eIF4G, NMD2 and CBP80"
/db_xref="CDD:LOAD_nic"
BASE COUNT 965 a 1323 c 1322 g 774 t
ORIGIN
1 ctcgaggcag gagtcggctc tccggagcct cgtccctccc ttccccttcc ctgccccctt
61 cccccacccc cgactcgggc ttggcgcggc ggccagagga accccgagcc cgcatttgct
121 gcatcgagca gttggtttag tatcagccaa acttgcagct gttcagaccg cgctccgccg
181 agtcccggcc caggcccctg agctggaggg atggaaaact cctctgcagc atcagcctcc
241 tcggaggcag ggagcagccg ctcccaggag atcgaggagc tggagcgctt catcgacagc
301 tacgtgctgg agtaccaggt gcaggggctg ctggctgaca agacggaggg tgatggcgag
361 agcgagagga cccagtccca catctcccag tggacagcgg actgcagcga accgctggac
421 agcagctgtt ccttctcccg agggcgagcc cccccacagc agaatggcag caaagacaac
481 tctctggaca tgctgggcac ggacatctgg gcggccaaca ccttcgattc cttcagtggt
541 gccacctggg acctgcagcc ggaaaagctg gacttcaccc agttccaccg caaagtccga
601 cacacgccca agcagcccct gccacacatc gaccgcgaag ggtgtggcaa agggaagctg
661 gaagatgggg atggcatcaa cctgaatgac atcgagaagg tccttccagc ctggcagggc
721 taccacccga tgccccatga agtggagatc gcacacacca agaagctgtt ccgcaggagg
781 agaaatgatc gaaggcggca gcagagacct ccggggggca acaagcccca acagcatggt
841 gaccaccagc caggcagtgc caaacacaac agggaccacc agaaatccta ccaggggggc
901 tcagcacccc acccctcagg gaggcccact caccatggct acagccagaa ccggcgctgg
961 caccatggca acatgaagca cccaccaggc gacaaggggg aggcaggcgc acaccgcaat
1021 gccaaagaga ccatgaccat cgagaaccca aaactggagg acactgcagg ggacaccggg
1081 cacagcagcc tcgaggcccc ccgcagccct gacaccctgg ccccggtggc ttctgagcgg
1141 ctgcccccac agcagtcagg ggggccagag gttgagacaa aacgtaaaga cagtattctt
1201 cccgagcgca tcggggagcg gcccaaaatt accctgctcc agtcttccaa agacagactg
1261 cggcgaaggc taaaggaaaa ggtaccggat gaagtggccg tggagacgac cactccccag
1321 cagaacaaga tggacaagct gatcgagatc ctgaacagca tgcggaacaa cagcagcgac
1381 gtggacacca agctcaccac cttcatggag gaggcccaga actccaccaa ctccgaggag
1441 atgctgggcg agatcgtgcg cacaatctac cagaaggctg tgtccgaccg cagcttcgcc
1501 ttcaccgctg ccaagctctg cgacaagatg gcgctcttta tggtggaggg gaccaagttc
1561 cggagcctgc tcctcaacat gctgcagaag gacttcacgg tgcgcgagga gctgcagcag
1621 caggacgtgg agcgctggct gggcttcatc accttcctgt gcgaggtctt cggcaccatg
1681 cgcagcagca caggcgagcc cttccgtgtg ctcgtgtgcc ccatctacac ctgcctcagg
1741 gagctcttgc aatctcagga tgtgaaggaa gatgctgtcc tttgctgctc tatggagctg
1801 cagagtacag gccggctgct ggaggaacag ctgcctgaga tgatgacaga gctcctggcc
1861 agcgcacggg acaagatgct gtgcccctcg gagtccatgc tgacccggtc gctgctccta
1921 gaggtcatcg agctccacgc taacagctgg aaccctctga cgccccccat cacgcagtac
1981 tacaacagaa ccatccagaa actgacagcc tgacagccag ggggcctggc gggcggccca
2041 cgggcagctg gggccctggt gcacagggcc agatggacag gcgggaggac aggggtggcc
2101 ctggcgggag aaagaaatgg ggaggagggc aggcagagtc ggtggccagt ctggagccag
2161 acggggaagg gagcaaatcc ctgagaggag tgcccccgca caagcccccc agcccgagca
2221 tgcaagctca caccaataag ggaagcatgt ttctttttcc tggtggccct ggccctcccc
2281 ttcctcactc ccgcctctcc cctccccatc agacccatcc cccacggagc tttgtgtgag
2341 ggatctcatc gctgtgactc ctcggagacc ttggcagcct cgcacgccgg ggcaccgctt
2401 gggtcagaaa ggacctcgga aggctgaaaa agtgggtcgg agacgggctc gcattgttcc
2461 cgcatgctgt cagccgcagt cgccaactgg cagcaggcga cgtgtagcag atgtccggga
2521 ggacaaaggc aggcacggtc cccaccagcc gcccgtaatt gacggccttt gtcagccatg
2581 gcagagctga cgctccacct cccacctcca agtcctcctc actgcagccc ccacagcctc
2641 aggcctaggg ggtcaggcgc agcgggggag atggagtttg cagttccact tgcactcttt
2701 tgtttattgt gttttatttt tcaaaagtcg gttgctttga agtctctttg gccaatgaaa
2761 atgcccgtga tgtgatcaca cagtcagcac tgttgaggac ccccggatta gtgggagatc
2821 aaacccagct cccctctaga agaaggattc gagccacaga cagcttgcca gtagccaatt
2881 agggtaattg gaaacttctg ccccggcggg gggtccccgc tggaatcctg tgttcctcgc
2941 cactggcttc cagcgcctct gttttctcaa agggctgata ctgtcaccac tgggaccaag
3001 ttaaacctgg tcctggcccc aggggccttg tggcaaacag ggcacagaac gagactggca
3061 aattaaaacc aaaattctag atggtgtctt gcgctccaca cgcaggtctt actggggaaa
3121 aggatgggag tgggggctcc ccaggactcg attttagcta atgcgctgtg tcactgcccc
3181 agctcggacg tagaagccca gccctccgtg agctcttggg aaaggggtga attcactggg
3241 tcatggaagg gacagtcagg tgaccagcgg ggtcgccaga tgaagcttcc cagccgggaa
3301 acaagacggg gtttcttggc aggccctggt cctggggagc aggccctgtt gttggctgga
3361 gaggaaggtg tggggtggaa caggtgtcca catagctcca tctctggggg ctggagcaca
3421 cactttgatg agcccccccg gaaatgatgt cagagcctag ccgcttcctt atttgctctt
3481 ttattgaggc cgggcaggcc ctgggtcact ttggaggccc ctcttggtcc acactggact
3541 ggccgggagg tgatgggcgg ggaaggttct cgtgattgat tgattctgag tctgagagtg
3601 gcgagtgggg agaggcttcc ccagttctct ccagctttcc ctgcagctgc aacctgccct
3661 ctggtcccag gtgtggagcc tttgcctgtc tctaaaaaga gcctgttggc gacaaggtgt
3721 agggggcaca agtttacctg aaacaggtca gtggtctctc ccaagaagcg cacgccacct
3781 ctggtccctg gccctgaacc ctgccttctt cctccctcca cggtttcttc ccagactttc
3841 tcaagctcct cctcactgcc cttcctcccc agcccagcct gggaacacag atgccccgcg
3901 ggtaggaggc ctcgagggag gagccgggct gatgcggggc tgctcagggc aggccccagg
3961 gcgagcttgc cgtcgtggcc aggcagcctc cacctgtgct tcagtggccc ctgcccccct
4021 gaagcatgtg gggtttgtcc gctaggagga ggcaaggccc ccgaagagag gagagacctg
4081 ggagtgggag ctcaggtcag ggaggaggca ggggagtggg gtctcccaga cccaacggtg
4141 agctcagagc aagcttcacg caggacgctc cgaaacactg tgtggagggg gctgtgttgt
4201 gggcaccttg gggcctgatt ctccttcctc cgaacgggct ccttgatggc ctggccacag
4261 gggcagctcc ccattggctg ttaggaccag agtgtgaaga agaagtgaaa tataaatatg
4321 tatacatata taaatatatt tttaattaca tgtcgtgtca aaaaaaaaaa aaaaaaaaaa
4381 aaaa
//