LOCUS BC040239 3634 bp mRNA linear HUM 19-JUN-2006
DEFINITION Homo sapiens pyruvate dehydrogenase kinase, isozyme 4, mRNA (cDNA
clone MGC:5281 IMAGE:3047987), complete cds.
ACCESSION BC040239
VERSION BC040239.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3634)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 3634)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (27-NOV-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 3 Row: f Column: 13
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 33589822.
FEATURES Location/Qualifiers
source 1..3634
/db_xref="H-InvDB:HIT000052352"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:5281 IMAGE:3047987"
/tissue_type="Cervix, carcinoma"
/clone_lib="NIH_MGC_12"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..3634
/gene="PDK4"
/db_xref="GeneID:5166"
/db_xref="HGNC:HGNC:8812"
/db_xref="MIM:602527"
CDS 201..1436
/gene="PDK4"
/codon_start=1
/product="PDK4 protein"
/protein_id="AAH40239.1"
/db_xref="GeneID:5166"
/db_xref="HGNC:HGNC:8812"
/db_xref="MIM:602527"
/translation="MKAARFVLRSAGSLNGAGLVPREVEHFSRYSPSPLSMKQLLDFG
SENACERTSFAFLRQELPVRLANILKEIDILPTQLVNTSSVQLVKSWYIQSLMDLVEF
HEKSPDDQKALSDFVDTLIKVRNRHHNVVPTMAQGIIEYKDACTVDPVTNQNLQYFLD
RFYMNRISTRMLMNQHILIFSDSQTGNPSHIGSIDPNCDVVAVVQDAFECSRMLCDQY
YLSSPELKLTQVNGKFPDQPIHIVYVPSHLHHMLFELFKNAMRATVEHQENQPSLTPI
EVIVVLGKEDLTIKISDRGGGVPLRIIDRLFSYTYSTAPTPVMDNSRNAPLAGFGYGL
PISRLYAKYFQGDLNLYSLSGYGTDAIIYLKALSSESIEKLPVFNKSAFKHYQMSSEA
DDWCIPSREPKNLAKEVAM"
BASE COUNT 1140 a 689 c 738 g 1067 t
ORIGIN
1 actgcatctc cgactctctg cccagactct tcactccgcg gcaccctcaa accccagccc
61 aggccggggc gcacgagcca gccagcgcac ctgcagtcct cgcccggacg cgccgcgccc
121 cctcggaacc aggctctgct ccgagcagcc ttcgcccctc aagccagcca cagtccccgc
181 caggccgggt gggcgtcaag atgaaggcgg cccgcttcgt gctgcgcagc gctggctcgc
241 tcaacggcgc cggcctggtg ccccgagagg tggagcattt ctcgcgctac agcccgtccc
301 cgctgtccat gaagcagcta ctggactttg gttcagaaaa tgcatgtgaa agaacttctt
361 ttgcattttt gcgacaagaa ttgcctgtga gactcgccaa cattctgaag gaaattgata
421 tcctcccgac ccaattagta aatacctctt cagtgcaatt ggttaaaagc tggtatatac
481 agagcctgat ggatttggtg gaattccatg agaaaagccc agatgaccag aaagcattat
541 cagactttgt agatacactc atcaaagttc gaaatagaca ccataatgta gtccctacaa
601 tggcacaagg aatcatagag tataaagatg cctgtacagt tgacccagtc accaatcaaa
661 atcttcaata tttcttggat cgattttaca tgaaccgtat ttctactcgg atgctgatga
721 accagcacat tcttatattt agtgactcac agacaggaaa cccaagccac attggaagca
781 ttgatcctaa ctgtgatgtg gtagcagtgg tccaagatgc ctttgagtgt tcaaggatgc
841 tctgtgatca gtattattta tcatctccag aattaaagct tacacaagtg aatggaaaat
901 ttccagacca accaattcac atcgtgtatg ttccttctca cctccatcat atgctctttg
961 aactatttaa gaatgcaatg cgggcaacag ttgaacacca ggaaaatcag ccttccctta
1021 caccaataga ggttattgtt gtcttgggaa aagaagacct taccattaag atttcagaca
1081 gaggaggtgg tgttcccctg agaattattg accgcctctt tagttataca tactccactg
1141 caccaacgcc tgtgatggat aattcccgga atgctccttt ggctggtttt ggttacggct
1201 tgccaatttc tcgtctgtat gccaagtact ttcaaggaga tctgaatctc tactctttat
1261 caggatatgg aacagatgct atcatctact taaaggcttt gtcttctgag tctatagaaa
1321 aacttccagt ttttaacaag tcagccttca aacattatca gatgagctct gaggctgatg
1381 actggtgtat cccaagcagg gaaccaaaga acctggcaaa agaagtggcc atgtgaagag
1441 ggacactcag gacactttac gggatcaaag tgggtctgca ccagtgctgc ttcctgaatg
1501 tttgtgtgtg aacccttgtt tcctccaaaa caaacgacag caacgaaaac tccttaatca
1561 gaacactgat ccaatgagga atggagcttg tttctgtgac ccaggagaac ttagtgcaag
1621 actacaggag ttaacagatg gccagctcct tattttttaa tgtagaataa ctcctgagtt
1681 tatatcaaat cctgaagaaa taagcctcag ttttccatct gtttttgata cgaataagaa
1741 agggagtgag tgtgaagatg gtggttagca gtttcactag actgatattt taggcctctt
1801 gttcacatca aaagatattg gtgtcagaat accagcattt tcctgccatg caaaggatta
1861 aaacttagtt tacactatgt ggttacaaat atatgtcaat gtacattttg aacatattta
1921 tgtgctatgg aaggaaatgc tggtgactaa aataaggttt actctgaaag aggaggaatt
1981 ttattcaaag cattcaaaca ttttattcaa gtgtttcaaa attcaaagca ttgtattcaa
2041 agttgcagtg aaggcatcaa cttatgtaaa aactcagaag gaaggctcct ctgataaaaa
2101 cacagctcct ttattatgct gcttttcttg ttcactttac acactaagta aacacttatt
2161 gtcaggtgcc tagtcttgag tgaattgtta gatgtgcact gaactcggga tgttggggat
2221 tggagagaga gaattgccaa agtaacagca aaaatatctc ttactttgct ttgtttataa
2281 ataaattagt agattggaaa aactagtgtt agggaaagaa atcacatgtt cagagcctaa
2341 ttcagtagga agggcttttc tctaccctga aatgaaggta atccaaaggc atccattttc
2401 taggcttaaa agatatattt ttgatatatt taattatatt ctctacactc cagcattaat
2461 atgtctgttt aaaaattact aattctcaaa tggctcaaga acattagaat ttaagtacct
2521 tttagagtaa ttattttaag caaatagcct ggacgtaaga gattctcatg ccagcatgct
2581 ttcatttgtc agttgttgtg actgagagct aatgaatgac acctgaaatg catatggtat
2641 ttttgggaga gttaaggtat aatttgaaga ttggcagacc agtcgcgctg attactctta
2701 gagaagaaga aatggaaaaa tgaaagaagg caggaaggaa agaaaggatg caggaaggaa
2761 agaaaggata caggaagaga gggaagcaga aggcagtcat ttttctattt tccccacaaa
2821 ttatttcaaa aaaaatctgt attttctggg atatgtcatt ggcaagagga agaactggtg
2881 tttcgaaagc agtatggatt ctttaaatgc ctctcactct tacaagatag taggctttga
2941 gataataaac ttacccgtgt caattaacat ttaaactggc atatagaaaa aaaggaggat
3001 ttttctgcat tgtaaaataa tcagtatggt ttatatgttg aatttgacat ttgtgtgtaa
3061 tttcatggtg gcctagtgtt gtggtgcttc tggtaatggt aatagaagct caactatttt
3121 tttgtggatt tcagttttta tcatcagaag tcctagacag tgacatttct taatggtggg
3181 agtccagctc atgcatttct gatcatacaa aacagtttgc agtaggttat ctgtcatttc
3241 agttttttac tgaaatttga gctaaacatt tttacatgta atacttgtat ttaccaaaga
3301 tttaaatcag ttgattaatt aattaactca aatactgtga actatcttta aaacactaga
3361 aaaaagaaat gttagtatct caattacacc aactgtgcaa atgaactttg ataaaataga
3421 aataatctac attggccttt gtgaaatctg gggaagagct ttaggatttt agtagatgga
3481 tactgaatac tcaggccccc ttaaattatt aatgtataca ttgtgttttt gtctttatgc
3541 tatgtacaga gaaatgtgat aattttttat aataaatatt ttttatgatg ataaaaaaaa
3601 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa
//