LOCUS BC032861 2473 bp mRNA linear HUM 28-JUL-2006
DEFINITION Homo sapiens Wilms tumor 1, mRNA (cDNA clone MGC:33649
IMAGE:4827849), complete cds.
ACCESSION BC032861
VERSION BC032861.2
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2473)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2473)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (07-JUN-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT On Aug 25, 2003 this sequence version replaced BC032861.1.
Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: Miklos Palkovits, M.D., Ph.D.
cDNA Library Preparation: Michael J. Brownstein (NHGRI) & Shiraki
Toshiyuki and Piero Carninci (RIKEN)
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Institute for Systems Biology
http://www.systemsbiology.org
contact: amadan@systemsbiology.org
Anup Madan, Jessica Fahey, Erin Helton, Mark Ketteman, Anuradha
Madan, Stephanie Rodrigues, Amy Sanchez and Michelle Whiting
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 47 Row: a Column: 6.
FEATURES Location/Qualifiers
source 1..2473
/db_xref="H-InvDB:HIT000051085"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:33649 IMAGE:4827849"
/tissue_type="Testis"
/clone_lib="NIH_MGC_97"
/lab_host="DH10B"
/note="Vector: pBluescriptR"
gene 1..2473
/gene="WT1"
/gene_synonym="WAGR"
/gene_synonym="WIT-2"
/gene_synonym="WT33"
/db_xref="GeneID:7490"
/db_xref="HGNC:HGNC:12796"
/db_xref="MIM:607102"
CDS 274..1182
/gene="WT1"
/gene_synonym="WAGR"
/gene_synonym="WIT-2"
/gene_synonym="WT33"
/codon_start=1
/product="WT1 protein"
/protein_id="AAH32861.1"
/db_xref="GeneID:7490"
/db_xref="HGNC:HGNC:12796"
/db_xref="MIM:607102"
/translation="MEKGYSTVTFDGTPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSL
GEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGAT
LKGVAAGSSSSVKWTEGQSNHSTGYESDNHTTPILCGAQYRMHTHGVFRGIQDVRRVP
GVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKPYQCDFKDCER
RFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGEKPFSCRWPSCQK
KFARSDELVRHHNMHQRNMTKLQLAL"
BASE COUNT 651 a 576 c 615 g 631 t
ORIGIN
1 gctttcacca ctgcccctcc cggggggacc tgaaggagag ggtttgaggc cggtctttgc
61 ccgccgaggt ctgcgtgtcc ggtctgggag gaggcctagg agggctcgcg ggccacggca
121 tccttgggcc cgagttctgg ggtgcggacg gacgtctcga gagtgggtgc cgcgactcgg
181 gacccacggc cctcgccggg cacggacagt tgcggagcag ggctctgagg attgtgcagt
241 gccctgggtc cctgcctact cctgggctca ggaatggaga agggttacag cacggtcacc
301 ttcgacggga cgcccagcta cggtcacacg ccctcgcacc atgcggcgca gttccccaac
361 cactcattca agcatgagga tcccatgggc cagcagggct cgctgggtga gcagcagtac
421 tcggtgccgc ccccggtcta tggctgccac acccccaccg acagctgcac cggcagccag
481 gctttgctgc tgaggacgcc ctacagcagt gacaatttat accaaatgac atcccagctt
541 gaatgcatga cctggaatca gatgaactta ggagccacct taaagggagt tgctgctggg
601 agctccagct cagtgaaatg gacagaaggg cagagcaacc acagcacagg gtacgagagc
661 gataaccaca caacgcccat cctctgcgga gcccaataca gaatgcacac gcacggtgtc
721 ttcagaggca ttcaggatgt gcggcgtgtg cctggagtag ccccgactct tgtacggtcg
781 gcatctgaga ccagtgagaa acgccccttc atgtgtgctt acccaggctg caataagaga
841 tattttaagc tgtcccactt acagatgcac agcaggaagc acactggtga gaaaccatac
901 cagtgtgact tcaaggactg tgaacgaagg ttttctcgtt cagaccagct caaaagacac
961 caaaggagac atacaggtgt gaaaccattc cagtgtaaaa cttgtcagcg aaagttctcc
1021 cggtccgacc acctgaagac ccacaccagg actcatacag gtgaaaagcc cttcagctgt
1081 cggtggccaa gttgtcagaa aaagtttgcc cggtcagatg aattagtccg ccatcacaac
1141 atgcatcaga gaaacatgac caaactccag ctggcgcttt gaggggtctc cctcggggac
1201 cgttcagtgt cccaggcagc acagtgtgtg aactgctttc aagtctgact ctccactcct
1261 cctcactaaa aaggaaactt cagttgatct tcttcatcca acttccaaga caagataccg
1321 gtgcttctgg aaactaccag gtgtgcctgg aagagttggt ctctgccctg cctactttta
1381 gttgactcac aggccctgga gaagcagcta acaatgtctg gttagttaaa agcccattgc
1441 catttggtct ggattttcta ctgtaagaag agccatagct gatcatgtcc ccctgaccct
1501 tcccttcttt ttttatgctc gttttcgctg gggatggaat tattgtacca ttttctatca
1561 tggaatattt ataggccagg gcatgtgtat gtgtctgcta atgtaaactt tgtcatggtt
1621 tccatttact aacagcaaca gcaagaaata aatcagagag caaggcatcg ggggtgaatc
1681 ttgtctaaca ttcccgaggt cagccaggct gctaacctgg aaagcaggat gtagttctgc
1741 caggcaactt ttaaagctca tgcatttcaa gcagctgaag aaagaatcag aactaaccag
1801 tacctctgta tagaaatcta aaagaatttt accattcagt taattcaatg tgaacactgg
1861 cacactgctc ttaagaaact atgaagatct gagatttttt tgtgtatgtt tttgactctt
1921 ttgagtggta atcatatgtg tctttataga tgtacatacc tccttgcaca aatggagggg
1981 aattcatttt catcactggg agtgtcctta gtgtataaaa accatgctgg tatatggctt
2041 caagttgtaa aaatgaaagt gactttaaaa gaaaataggg gatggtccag gatctccact
2101 gataagactg tttttaagta acttaaggac ctttgggtct acaagtatat gtgaaaaaaa
2161 atgagactta ctgggtgagg aaatccattg tttaaagatg gtcgtgtgtg tgtgtgtgtg
2221 tgtgtgtgtg ttgtgttgtg ttttattttt taagggaggg aatttattat ttaccgttgc
2281 ttgaaattac tgtgtaaata tatgtctgtt aatgatttgc tctttgacaa ctaaaattag
2341 gactgtataa gtactagatg catcactggg tgttgatctt acaagatatt gatgataaca
2401 cttaaaattg taacctgcat ttttcacttt gctctcaatt aaagtctatt caaaaggaaa
2461 aaaaaaaaaa aaa
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