LOCUS BC028179 1424 bp mRNA linear HUM 15-JUL-2006
DEFINITION Homo sapiens eukaryotic translation elongation factor 1 gamma, mRNA
(cDNA clone MGC:40101 IMAGE:5402023), complete cds.
ACCESSION BC028179
VERSION BC028179.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1424)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1424)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (08-APR-2002) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: National Institutes of Health Intramural
Sequencing Center (NISC),
Gaithersburg, Maryland;
Web site: http://www.nisc.nih.gov/
Contact: nisc_mgc@nhgri.nih.gov
Akhter,N., Ayele,K., Beckstrom-Sternberg,S.M., Benjamin,B.,
Blakesley,R.W., Bouffard,G.G., Breen,K., Brinkley,C., Brooks,S.,
Dietrich,N.L., Granite,S., Guan,X., Gupta,J., Haghighi,P.,
Hansen,N., Ho,S.-L., Karlins,E., Kwong,P., Laric,P., Legaspi,R.,
Maduro,Q.L., Masiello,C., Maskeri,B., Mastrian,S.D.,McCloskey,J.C.,
McDowell,J., Pearson,R., Stantripop,S., Thomas,P.J., Touchman,J.W.,
Tsurgeon,C., Vogt,J.L., Walker,M.A., Wetherby,K.D., Wiggins,L.,
Young,A., Zhang,L.-H. and Green,E.D.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 62 Row: p Column: 15
This clone was selected for full length sequencing because it
passed the following selection criteria: Similarity but not
identity to protein.
FEATURES Location/Qualifiers
source 1..1424
/db_xref="H-InvDB:HIT000040479"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:40101 IMAGE:5402023"
/tissue_type="Liver, adenocarcinoma"
/clone_lib="NIH_MGC_90"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..1424
/gene="EEF1G"
/gene_synonym="EF1G"
/gene_synonym="GIG35"
/db_xref="GeneID:1937"
/db_xref="HGNC:HGNC:3213"
/db_xref="MIM:130593"
CDS 19..1332
/gene="EEF1G"
/gene_synonym="EF1G"
/gene_synonym="GIG35"
/codon_start=1
/product="eukaryotic translation elongation factor 1
gamma"
/protein_id="AAH28179.1"
/db_xref="GeneID:1937"
/db_xref="HGNC:HGNC:3213"
/db_xref="MIM:130593"
/translation="MAAGTLYTYPENWRAFKALIAAQYSGAQVRVLSAPPHFHFGQTN
RTPEFLRKFPAGKVPAFEGDDGFCVFESNAIAYYVSNEELRGSTPEAAAQVVQWVSFA
DSDIVPPASTWVFPTLGIMHHNKQATENAKEEVRRILGLLDAYLKTRTFLVGERVTLA
DITVVCTLLWLYKQVLEPSFRQAFPNTNRWFLTCINQPQFRAVLGEVKLCEKMAQFDA
KKFAETQPKKDTPRKEKGSREEKQKPQAERKEEKKAAAPAPEEEMDECEQALAAEPKA
KDPFAHLPKSTFVLDEFKRKYSNEDTLSVALPYFWEHFDKDGWSLWYSEYRFPEELTQ
TFMSCNLITGMFQRLDKLRKNAFASVILFGTNNSSSISGVWVFRGQELAFPLSPDWQV
DYESYTWRKLDPGSEETQTLVREYFSWEGAFQHVGKAFNQGKIFK"
BASE COUNT 349 a 375 c 392 g 308 t
ORIGIN
1 ccacgcgtcc gaatcaccat ggcggctggg accctgtaca cgtatcctga aaactggagg
61 gccttcaagg ctctcatcgc tgctcagtac agcggggctc aggtccgcgt gctctccgca
121 ccaccccact tccattttgg ccaaaccaac cgcacccctg aatttctccg caaatttcct
181 gccggcaagg tcccagcatt tgagggtgat gatggattct gtgtgtttga gagcaacgcc
241 attgcctact atgtgagcaa tgaggagctg cggggaagta ctccagaggc agcagcccag
301 gtggtgcagt gggtgagctt tgctgattcc gatatagtgc ccccagccag tacctgggtg
361 ttccccacct tgggcatcat gcaccacaac aaacaggcca ctgagaatgc aaaggaggaa
421 gtgaggcgaa ttctggggct gctggatgct tacttgaaga cgaggacttt tctggtgggc
481 gaacgagtga cattggctga catcacagtt gtctgcaccc tgttgtggct ctataagcag
541 gttctagagc cttctttccg ccaggccttt cccaatacca accgctggtt cctcacctgc
601 attaaccagc cccagttccg ggctgtcttg ggcgaagtga aactgtgtga gaagatggcc
661 cagtttgatg ctaaaaagtt tgcagagacc caacctaaaa aggacacacc acggaaagag
721 aagggttcac gggaagagaa gcagaagccc caggctgagc ggaaggagga gaaaaaggcg
781 gctgcccctg ctcctgagga ggagatggat gaatgtgagc aggcgctggc tgctgagccc
841 aaggccaagg accccttcgc tcacctgccc aagagtacct ttgtgttgga tgaatttaag
901 cgcaagtact ccaatgagga cacactctct gtggcactgc catatttctg ggagcacttt
961 gataaggacg gctggtccct gtggtactca gagtatcgct tccctgaaga actcactcag
1021 accttcatga gctgcaatct catcactgga atgttccagc gactggacaa gctgaggaag
1081 aatgccttcg ccagtgtcat cctttttgga accaacaata gcagctccat ttctggagtc
1141 tgggtcttcc gaggccagga gcttgccttt ccgctgagtc cagattggca ggtggactac
1201 gagtcataca catggcggaa actggatcct ggcagcgagg agacccagac gctggttcga
1261 gagtactttt cctgggaggg ggccttccag catgtgggca aagccttcaa tcagggcaag
1321 atcttcaagt gaacatctct tgccatcacc tagctgcctg cacctgccct tcagggagat
1381 gggggtcatt aaaggaaact gaacattgaa aaaaaaaaaa aaaa
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