LOCUS BC016184 1879 bp mRNA linear HUM 24-JUL-2006
DEFINITION Homo sapiens ER degradation enhancer, mannosidase alpha-like 2,
mRNA (cDNA clone MGC:23193 IMAGE:4860424), complete cds.
ACCESSION BC016184
VERSION BC016184.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1879)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 1879)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (29-OCT-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC/DCTD/DTP
cDNA Library Preparation: Rubin Laboratory
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Genome Sequence Centre,
BC Cancer Agency, Vancouver, BC, Canada
info@bcgsc.bc.ca
Martin Hirst, Thomas Zeng, Ryan Morin, Michelle Moksa, Johnson
Pang, Diana Mah, Jing Wang, Kieth Fichter, Eric Chuah, Allen
Delaney, Rob Kirkpatrick, Agnes Baross, Sarah Barber, Mabel
Brown-John, Steve S. Chand, William Chow, Ryan Babakaiff, Dave
Wong, Corey Matsuo, Jaclyn Beland, Susan Gibson, Luis delRio, Ruth
Featherstone, Malachi Griffith, Obi Griffith, Ran Guin, Nancy Liao,
Kim MacDonald, Mike R. Mayo, Josh Moran, Diana Palmquist, JR
Santos, Duane Smailus, Jeff Stott, Miranda Tsai, George Yang,
Jacquie Schein, Asim Siddiqui,Steven Jones, Rob Holt, Marco Marra.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAL Plate: 34 Row: g Column: 4
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 8922666.
FEATURES Location/Qualifiers
source 1..1879
/db_xref="H-InvDB:HIT000037502"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:23193 IMAGE:4860424"
/tissue_type="Skin, melanotic melanoma, high MDR."
/clone_lib="NIH_MGC_49"
/lab_host="DH10B-R"
/note="Vector: pOTB7"
gene 1..1879
/gene="EDEM2"
/gene_synonym="bA4204.1"
/gene_synonym="FLJ10783"
/db_xref="GeneID:55741"
/db_xref="HGNC:HGNC:15877"
CDS 42..1778
/gene="EDEM2"
/gene_synonym="bA4204.1"
/gene_synonym="FLJ10783"
/codon_start=1
/product="ER degradation enhancer, mannosidase alpha-like
2"
/protein_id="AAH16184.1"
/db_xref="GeneID:55741"
/db_xref="HGNC:HGNC:15877"
/translation="MPFRLLIPLGLLCALLPQHHGAPGPDGSAPDPAHYRERVKAMFY
HAYDSYLENAFPFDELRPLTCDGHDTWGSFSLTLIDALDTLLILGNVSEFQRVVEVLQ
DSVDFDIDVNASVFETNIRVVGGLLSAHLLSKKAGVEVEAGWPCSGPLLRMAEEAARK
LLPAFQTPTGMPYGTVNLLHGVNPGETPVTCTAGIGTFIVEFATLSSLTGDPVFEDVA
RVALMRLWESRSDIGLVGNHIDVLTGKWVAQDAGIGAGVDSYFEYLVKGAILLQDKKL
MAMFLEYNKAIRNYTRFDDWYLWVQMYKGTVSMPVFQSLEAYWPGLQSLIGDIDNAMR
TFLNYYTVWKQFGGLPEFYNIPQGYTVEKREGYPLRPELIESAMYLYRATGDPTLLEL
GRDAVESIEKISKVECGFATIKDLRDHKLDNRMESFFLAETVKYLYLLFDPTNFIHNN
GSTFDTVITPYGECILGAGGYIFNTEAHPIDPAALHCCQRLKEEQWEVEDLMREFYSL
KRSRSKFQKNTVSSGPWEPPARPGTLFSPENHDQARERKPAKQKVPLLSCPSQPFTSK
LALLGQVFLDSS"
BASE COUNT 458 a 505 c 497 g 419 t
ORIGIN
1 ggctgctgct gctgcggggg cgctgagagg acacgagctc tatgcctttc cggctgctca
61 tcccgctcgg cctcctgtgc gcgctgctgc ctcagcacca tggtgcgcca ggtcccgacg
121 gctccgcgcc agatcccgcc cactacaggg agcgagtcaa ggccatgttc taccacgcct
181 acgacagcta cctggagaat gcctttccct tcgatgagct gcgacctctc acctgtgacg
241 ggcacgacac ctggggcagt ttttctctga ctctaattga tgcactggac accttgctga
301 ttttggggaa tgtctcagaa ttccaaagag tggttgaagt gctccaggac agcgtggact
361 ttgatattga tgtgaacgcc tctgtgtttg aaacaaacat tcgagtggta ggaggactcc
421 tgtctgctca tctgctctcc aagaaggctg gggtggaagt agaggctgga tggccctgtt
481 ccgggcctct cctgagaatg gctgaggagg cggcccgaaa actcctccca gcctttcaga
541 cccccactgg catgccatat ggaacagtga acttacttca tggcgtgaac ccaggagaga
601 cccctgtcac ctgtacggca gggattggga ccttcattgt tgaatttgcc accctgagca
661 gcctcactgg tgacccggtg ttcgaagatg tggccagagt ggctttgatg cgcctctggg
721 agagccggtc agatatcggg ctggtcggca accacattga tgtgctcact ggcaagtggg
781 tggcccagga cgcaggcatc ggggctggcg tggactccta ctttgagtac ttggtgaaag
841 gagccatcct gcttcaggat aagaagctca tggccatgtt cctagagtat aacaaagcca
901 tccggaacta cacccgcttc gatgactggt acctgtgggt tcagatgtac aaggggactg
961 tgtccatgcc agtcttccag tccttggagg cctactggcc tggtcttcag agcctcattg
1021 gagacattga caatgccatg aggaccttcc tcaactacta cactgtatgg aagcagtttg
1081 gggggctccc ggaattctac aacattcctc agggatacac agtggagaag cgagagggct
1141 acccacttcg gccagaactt attgaaagcg caatgtacct ctaccgtgcc acgggggatc
1201 ccaccctcct agaactcgga agagatgctg tggaatccat tgaaaaaatc agcaaggtgg
1261 agtgcggatt tgcaacaatc aaagatctgc gagaccacaa gctggacaac cgcatggagt
1321 cgttcttcct ggccgagact gtgaaatacc tctacctcct gtttgaccca accaacttca
1381 tccacaacaa tgggtccacc ttcgacacgg tgatcacccc ctatggggag tgcatcctgg
1441 gggctggggg gtacatcttc aacacagaag ctcaccccat cgaccctgcc gccctgcact
1501 gctgccagag gctgaaggaa gagcagtggg aggtggagga cttgatgagg gaattctact
1561 ctctcaaacg gagcaggtcg aaatttcaga aaaacactgt tagttcgggg ccatgggaac
1621 ctccagcaag gccaggaaca ctcttctcac cagaaaacca tgaccaggca agggagagga
1681 agcctgccaa acagaaggtc ccacttctca gctgccccag tcagcccttc acctccaagt
1741 tggcattact gggacaggtt ttcctagact cctcataacc actggataat ttttttattt
1801 ttattttttt gaggctaaac tataataaat tgcttttggc tatcataaaa aaaaaaaaaa
1861 aaaaaaaaaa aaaaaaaaa
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