LOCUS BC010578 2662 bp mRNA linear HUM 11-AUG-2006
DEFINITION Homo sapiens threonyl-tRNA synthetase, mRNA (cDNA clone MGC:9344
IMAGE:3458845), complete cds.
ACCESSION BC010578
VERSION BC010578.1
KEYWORDS MGC.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2662)
AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
CONSRTM Mammalian Gene Collection Program Team
TITLE Generation and initial analysis of more than 15,000 full-length
human and mouse cDNA sequences
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
PUBMED 12477932
REFERENCE 2 (bases 1 to 2662)
CONSRTM NIH MGC Project
TITLE Direct Submission
JOURNAL Submitted (10-JUL-2001) National Institutes of Health, Mammalian
Gene Collection (MGC), Bethesda, MD 20892-2590, USA
REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT Contact: MGC help desk
Email: cgapbs-r@mail.nih.gov
Tissue Procurement: ATCC
cDNA Library Preparation: Life Technologies, Inc.
cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
DNA Sequencing by: Baylor College of Medicine Human Genome
Sequencing Center
Center code: BCM-HGSC
Web site: http://www.hgsc.bcm.tmc.edu/cdna/
Contact: amg@bcm.tmc.edu
Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H.,
Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati,
A.N., Gibbs, R.A.
Clone distribution: MGC clone distribution information can be found
through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
Series: IRAK Plate: 12 Row: a Column: 19
This clone was selected for full length sequencing because it
passed the following selection criteria: matched mRNA gi: 38202254.
FEATURES Location/Qualifiers
source 1..2662
/db_xref="H-InvDB:HIT000035007"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/clone="MGC:9344 IMAGE:3458845"
/tissue_type="Cervix, carcinoma"
/clone_lib="NIH_MGC_12"
/lab_host="DH10B"
/note="Vector: pCMV-SPORT6"
gene 1..2662
/gene="TARS"
/gene_synonym="MGC9344"
/gene_synonym="ThrRS"
/db_xref="GeneID:6897"
/db_xref="HGNC:HGNC:11572"
/db_xref="MIM:187790"
CDS 99..2270
/gene="TARS"
/gene_synonym="MGC9344"
/gene_synonym="ThrRS"
/codon_start=1
/product="threonyl-tRNA synthetase"
/protein_id="AAH10578.2"
/db_xref="GeneID:6897"
/db_xref="HGNC:HGNC:11572"
/db_xref="MIM:187790"
/translation="MFEEKASSPSGKMGGEEKPIGAGEEKQKEGGKKKNKEGSGDGGR
AELNPWPEYIYTRLEMYNILKAEHDSILAEKAEKDSKPIKVTLPDGKQVDAESWKTTP
YQIACGISQGLADNTVIAKVNNVVWDLDRPLEEDCTLELLKFEDEEAQAVYWHSSAHI
MGEAMERVYGGCLCYGPPIENGFYYDMYLEEGGVSSNDFSSLEALCKKIIKEKQAFER
LEVKKETLLAMFKYNKFKCRILNEKVNTPTTTVYRCGPLIDLCRGPHVRHTGKIKALK
IHKNSSTYWEGKADMETLQRIYGISFPDPKMLKEWEKFQEEAKNRDHRKIGRDQELYF
FHELSPGSCFFLPKGAYIYNALIEFIRSEYRKRGFQEVVTPNIFNSRLWMTSGHWQHY
SENMFSFEVEKELFALKPMNCPGHCLMFDHRPRSWRELPLRLADFGVLHRNELSGALT
GLIRVRRFQQDDAHIFCAMEQIEDEIKGCLDFLRTVYSVFGFSFKLNLSTRPEKFLGD
IEVWDQAEKQLENSLNEFGEKWELNSGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQ
LPIRFNLTYVSHDGDDKKRPVIVHRAILGSVERMIAILTENYGGKWPFWLSPRQVMVV
PVGPTCDEYAQKVRQQFHDAKFMADIDLDPGCTLNKKIRNAQLAQYNFILVVGEKEKI
SGTVNIRTRDNKVHGERTISETIERLQQLKEFRSKQAEEEF"
BASE COUNT 852 a 499 c 631 g 680 t
ORIGIN
1 ggccaagtcc cgggcgctag cccacctccc acccgcctct tggctcctct cctctaggcc
61 gtcgctttcg ggttctctca tcgcttcgtc gttcgccaat gtttgaggag aaggccagca
121 gtccttcagg gaagatggga ggcgaggaga agccgattgg tgctggtgaa gagaagcaaa
181 aggaaggagg caaaaagaag aacaaagaag gatctggaga tggaggtcga gctgagttga
241 atccttggcc tgaatatatt tacacacgtc ttgagatgta taatatacta aaagcagaac
301 atgattccat tctggcagaa aaggcagaaa aagatagcaa gccaattaaa gtcactttgc
361 ctgatggtaa acaggttgat gcggaatctt ggaaaactac accatatcaa attgcctgtg
421 gaattagtca aggcctggcc gacaacaccg ttattgctaa agtaaataat gttgtgtggg
481 acctggaccg ccctctggaa gaagattgta ccttggagct tctcaagttt gaggatgagg
541 aagctcaggc agtgtattgg cactctagtg ctcacataat gggtgaagcc atggaaagag
601 tctatggtgg atgtttatgc tacggtccgc caatagaaaa tggattctat tatgacatgt
661 acctcgaaga agggggtgtg tctagcaatg atttctcttc tctggaggct ttgtgtaaga
721 aaatcattaa agaaaaacaa gcttttgaaa gactggaagt taagaaagaa actttactgg
781 caatgtttaa gtacaacaag ttcaaatgcc ggatattgaa tgaaaaggtg aatactccaa
841 ctaccacagt ctatagatgt ggccctttga tagatctctg ccggggtcct catgttagac
901 acacgggcaa aattaaggct ttaaaaatac acaaaaattc ctccacgtac tgggaaggca
961 aagcagatat ggagactctc cagagaattt atggcatttc attcccagat cctaaaatgt
1021 tgaaagagtg ggagaagttc caagaggaag ctaaaaaccg agatcatagg aaaattggca
1081 gggaccaaga actatatttc tttcatgaac tcagccctgg aagttgcttt tttctgccaa
1141 aaggagccta catttataat gcacttattg aattcattag gagcgaatat aggaaaagag
1201 gattccagga ggtagtcacc ccaaacatct tcaacagccg actctggatg acctcgggcc
1261 actggcagca ctacagcgag aacatgttct cctttgaggt ggagaaggag ctgtttgccc
1321 tgaaacccat gaactgccca ggacactgcc ttatgtttga tcatcggcca aggtcctggc
1381 gagaactgcc tctgcggcta gctgattttg gggtacttca taggaacgag ctgtctggag
1441 cactcacagg actcatccgg gtacgaagat tccaacagga tgatgctcac atattctgtg
1501 ccatggagca gattgaagat gaaataaaag gttgtttgga ttttctacgt acggtatata
1561 gcgtatttgg attttctttt aaactaaacc tttctactcg cccggaaaaa ttccttggag
1621 atatcgaagt atgggatcaa gctgagaaac aacttgaaaa cagtctgaat gaatttggtg
1681 aaaagtggga gttaaactct ggagatggag ctttctatgg cccaaagatt gacatacaga
1741 ttaaagatgc gattgggcgg taccaccagt gtgcaaccat ccagctggat ttccagttgc
1801 ccatcagatt taatcttact tatgtaagcc atgatggtga tgataagaaa aggccagtga
1861 ttgttcatcg agccatcttg ggatcagtgg aaagaatgat tgctatcctc acagaaaact
1921 atgggggcaa atggcccttt tggctgtccc ctcgccaggt aatggtagtt ccagtgggac
1981 caacctgtga tgaatatgcc caaaaggtac gacaacaatt ccacgatgcc aaattcatgg
2041 cagacattga tctggatcca ggctgtacat tgaataaaaa gattcgaaat gcacagttag
2101 cacagtataa cttcatttta gttgttggtg aaaaagagaa aatcagtggc actgttaata
2161 tccgcacaag agacaataag gtccacgggg aacgcaccat ttctgaaact atcgagcggc
2221 tacagcagct caaagagttc cgcagcaaac aggcagaaga agaattttaa tgaaaaaatt
2281 acccagattg gctccatgga aaaggaggaa cagcgtttcc gtaaaattga ctttgtactc
2341 tgaaaacgtc aatttatatt gaacttggag gagtttggca aagtccgaat aggtcaacct
2401 gcaggcgtaa ctatttttga cctagtcagt ttttaaacaa tgtgcatttg aaggagttaa
2461 ttaaaagaga gccaataaaa tgattttact cattcagtat ctgagtactg gaagtgaaac
2521 atgaggaatg ctttagtgta atgtgggaga acttttttgt aaatttaatg caattgaaaa
2581 agttttcaaa ttcaattaag ataactagaa ttggattatg gtgtaaaaat aaaaaaaaaa
2641 tttattcaaa aaaaaaaaaa aa
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