LOCUS       AI060807                 501 bp    mRNA    linear   EST 22-JUL-1998
DEFINITION  ub44b05.r1 Soares_thymus_2NbMT Mus musculus cDNA clone
            IMAGE:1380561 5' similar to gb:U05252 Mus musculus nuclear matrix
            attachment DNA-binding protein SATB1 (MOUSE), mRNA sequence.
ACCESSION   AI060807
VERSION     AI060807.1
DBLINK      BioSample: SAMN00155149
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Marra,M., Hillier,L., Allen,M., Bowles,M., Dietrich,N., Dubuque,T.,
            Geisel,S., Kucaba,T., Lacy,M., Le,M., Martin,J., Morris,M.,
            Schellenberg,K., Steptoe,M., Tan,F., Underwood,K., Moore,B.,
            Theising,B., Wylie,T., Lennon,G., Soares,B., Wilson,R. and
            Waterston,R.
  TITLE     The WashU-HHMI Mouse EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Marra M/Mouse EST Project
            WashU-HHMI Mouse EST Project
            Washington University School of MedicineP
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: mouseest@watson.wustl.edu
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            MGI:903029
            Seq primer: -28m13 rev2 ET from Amersham
            High quality sequence stop: 494.
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="taxon:10090"
                     /clone="IMAGE:1380561"
                     /sex="male"
                     /tissue_type="Thymus"
                     /clone_lib="SAMN00155149 Soares_thymus_2NbMT"
                     /dev_stage="4 weeks"
                     /lab_host="DH10B"
                     /note="Vector: pT7T3D-PacI; Site_1: Not I; Site_2: Eco RI;
                     1st strand cDNA was primed with a Not I - oligo(dT) primer
                     [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCGTTTTTTTTTTTTTTTTTTTTTTTTT
                     3']; double-stranded cDNA was ligated to Eco RI adaptors
                     (Pharmacia), digested with Not I and cloned into the Not I
                     and Eco RI sites of the modified pT7T3 vector. RNA
                     provided by Dr. Bertrand Jordan. Library went through two
                     rounds of normalization, and was constructed by Bento
                     Soares and M.Fatima Bonaldo."
BASE COUNT          114 a          137 c          109 g          141 t
ORIGIN      
        1 cacagggcat ctgtcacata agacagtgga actggattcc acttcactac ctggatgagc
       61 cctttggctt gggcagcaga gtgtgttgaa tagcctagag acagcaacgt atctctatca
      121 gctggttgaa aagcatatcc tttctcacca atacaaattc cgcgtgctcc tccttgcaat
      181 catactcaat ggcgttttca taatgttcca ccacgcagaa aactggtaac atggttcctt
      241 tcctaaggtt ggttttcatg agatggcccg agtgttttaa aggcactccc tgcatctttc
      301 cacctgtgct cccaagcctt cctcttccta gagggctccc gttctgctcc aggcgggcaa
      361 tcttggcggg tggaccttcg gatcactcac attgttagac atttctgaat gttctttcgc
      421 ctgagttgcc tcgttcaaat gatccatact cagtcactgt cttacagatc acctgccaga
      481 atcaggcgtg gaaccgggac a
//