LOCUS AMU96404.1 533 aa PRT BCT 21-JUN-2016
DEFINITION Sphingopyxis terrae subsp. terrae NBRC 15098 lysine--tRNA
ligase protein.
ACCESSION CP013342-3439
PROTEIN_ID AMU96404.1
SOURCE Sphingopyxis terrae subsp. terrae NBRC 15098
ORGANISM Sphingopyxis terrae subsp. terrae NBRC 15098
Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
Sphingomonadaceae; Sphingopyxis.
REFERENCE 1 (bases 1 to 3979087)
AUTHORS Ohtsubo,Y., Nonoyama,S., Nagata,Y., Numata,M., Tsuchikane,K.,
Hosoyama,A., Yamazoe,A., Tsuda,M., Fujita,N. and Kawai,F.
TITLE Complete Genome Sequence of Sphingopyxis terrae Strain 203-1 (NBRC
111660), a Polyethylene Glycol Degrader
JOURNAL Genome Announc 4 (3) (2016)
PUBMED 27284143
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 3979087)
AUTHORS Yoshiyuki,O.
TITLE Direct Submission
JOURNAL Submitted (30-NOV-2015) Department of Life Sciences, Tohoku
University, Katahira 2-1-1, Sendai 980-8577, Japan
COMMENT Annotation was added by the NCBI Prokaryotic Genome Annotation
Pipeline (released 2013). Information about the Pipeline can be
found here: http://www.ncbi.nlm.nih.gov/genome/annotation_prok/
Annotation was conducted using PGAP and MiGAP, and PGAP annotation
was curated by referencing to the MiGAP annotation with the aid of
annotation support tool of GenomeMatcher software.
##Genome-Assembly-Data-START##
Assembly Method :: newbler v. 2.8
Genome Coverage :: 110x
Sequencing Technology :: Illumina MiSeq
##Genome-Assembly-Data-END##
##Genome-Annotation-Data-START##
Annotation Provider :: NCBI
Annotation Date :: 11/30/2015 14:33:14
Annotation Pipeline :: NCBI Prokaryotic Genome Annotation
Pipeline
Annotation Method :: Best-placed reference protein set;
GeneMarkS+
Annotation Software revision :: 3.0
Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA;
repeat_region
Genes :: 3,797
CDS :: 3,699
Pseudo Genes :: 49
rRNAs :: 1, 1, 1 (5S, 16S, 23S)
complete rRNAs :: 1, 1, 1 (5S, 16S, 23S)
tRNAs :: 45
ncRNAs :: 1
##Genome-Annotation-Data-END##
FEATURES Qualifiers
source /organism="Sphingopyxis terrae subsp. terrae NBRC 15098"
/mol_type="genomic DNA"
/strain="203-1"
/sub_species="terrae"
/culture_collection="NBRC:15098"
/type_material="type strain of Sphingomonas terrae"
/db_xref="taxon:1219058"
/geo_loc_name="Japan: Kyoto"
/collection_date="1974"
protein /gene="lysK"
/locus_tag="AOA14_17530"
/EC_number="6.1.1.6"
/inference="EXISTENCE: similar to AA
sequence:RefSeq:WP_011540775.1"
/note="class I; LysRS1; catalyzes a two-step reaction,
first charging a lysine molecule by linking its carboxyl
group to the alpha-phosphate of ATP, followed by transfer
of the aminoacyl-adenylate to its tRNA; in Methanosarcina
barkeri this enzyme charges both tRNA molecules for lysine
that exist in this organism (but the tRNALysUUU very
poorly) and in the presence of LysRS2 can charge tRNAPyl
with lysine; Derived by automated computational analysis
using gene prediction method: Protein Homology."
/transl_table=11
BEGIN
1 MTDLHLYPSL REPAQTSKAW PFEEARKLIK RYPDGKPGGA PVLFETGYGP SGLPHIGTFN
61 EVLRTTMVRR AYETLTGGAP TRLVAFSDDM DGLRKVPDNV PNGDMLREHL GRPLTAIPDP
121 FGKYESFAHH NNAMLREFLD RFGFEYEFVS STERYRSGAF DDALKNVLRH NQDILDIMLP
181 TLRAERAATY SPILPVSPKS GIVLQVPVTV VDPDAGLVSF EDEGETITHS ILSGGAKLQW
241 KVDWAMRWVA LGVDYEMYGK DLTDSGIQSG KIAKALGGRK PEGLIYEMFL DEKGEKISKS
301 KGNGLSIEQW LSYGSEESLA FYAYREPKAA KQLHIGVIPK AVDEYLQMRG SYPAQEPDKK
361 LGNPVHHVHV ARGQDVPDAV LPVTFGLLLN LVAVMGADAA KDQIWRYLGQ YVAGADAATW
421 PELDRLIDNA ITYNRDYVAP TLKRRKPVGG EGAALKELDG RLAALPADAS ADDIQNIVYE
481 IGKNEAYGFE NLRDWFKALY ETLLGSSAGP RMGSFIALFG IDNTRRLIAE ALA
//