LOCUS AMU96404.1 533 aa PRT BCT 21-JUN-2016 DEFINITION Sphingopyxis terrae subsp. terrae NBRC 15098 lysine--tRNA ligase protein. ACCESSION CP013342-3439 PROTEIN_ID AMU96404.1 SOURCE Sphingopyxis terrae subsp. terrae NBRC 15098 ORGANISM Sphingopyxis terrae subsp. terrae NBRC 15098 Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingopyxis. REFERENCE 1 (bases 1 to 3979087) AUTHORS Ohtsubo,Y., Nonoyama,S., Nagata,Y., Numata,M., Tsuchikane,K., Hosoyama,A., Yamazoe,A., Tsuda,M., Fujita,N. and Kawai,F. TITLE Complete Genome Sequence of Sphingopyxis terrae Strain 203-1 (NBRC 111660), a Polyethylene Glycol Degrader JOURNAL Genome Announc 4 (3) (2016) PUBMED 27284143 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 3979087) AUTHORS Yoshiyuki,O. TITLE Direct Submission JOURNAL Submitted (30-NOV-2015) Department of Life Sciences, Tohoku University, Katahira 2-1-1, Sendai 980-8577, Japan COMMENT Annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (released 2013). Information about the Pipeline can be found here: http://www.ncbi.nlm.nih.gov/genome/annotation_prok/ Annotation was conducted using PGAP and MiGAP, and PGAP annotation was curated by referencing to the MiGAP annotation with the aid of annotation support tool of GenomeMatcher software. ##Genome-Assembly-Data-START## Assembly Method :: newbler v. 2.8 Genome Coverage :: 110x Sequencing Technology :: Illumina MiSeq ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 11/30/2015 14:33:14 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline Annotation Method :: Best-placed reference protein set; GeneMarkS+ Annotation Software revision :: 3.0 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes :: 3,797 CDS :: 3,699 Pseudo Genes :: 49 rRNAs :: 1, 1, 1 (5S, 16S, 23S) complete rRNAs :: 1, 1, 1 (5S, 16S, 23S) tRNAs :: 45 ncRNAs :: 1 ##Genome-Annotation-Data-END## FEATURES Qualifiers source /organism="Sphingopyxis terrae subsp. terrae NBRC 15098" /mol_type="genomic DNA" /strain="203-1" /sub_species="terrae" /culture_collection="NBRC:15098" /type_material="type strain of Sphingomonas terrae" /db_xref="taxon:1219058" /geo_loc_name="Japan: Kyoto" /collection_date="1974" protein /gene="lysK" /locus_tag="AOA14_17530" /EC_number="6.1.1.6" /inference="EXISTENCE: similar to AA sequence:RefSeq:WP_011540775.1" /note="class I; LysRS1; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri this enzyme charges both tRNA molecules for lysine that exist in this organism (but the tRNALysUUU very poorly) and in the presence of LysRS2 can charge tRNAPyl with lysine; Derived by automated computational analysis using gene prediction method: Protein Homology." /transl_table=11 BEGIN 1 MTDLHLYPSL REPAQTSKAW PFEEARKLIK RYPDGKPGGA PVLFETGYGP SGLPHIGTFN 61 EVLRTTMVRR AYETLTGGAP TRLVAFSDDM DGLRKVPDNV PNGDMLREHL GRPLTAIPDP 121 FGKYESFAHH NNAMLREFLD RFGFEYEFVS STERYRSGAF DDALKNVLRH NQDILDIMLP 181 TLRAERAATY SPILPVSPKS GIVLQVPVTV VDPDAGLVSF EDEGETITHS ILSGGAKLQW 241 KVDWAMRWVA LGVDYEMYGK DLTDSGIQSG KIAKALGGRK PEGLIYEMFL DEKGEKISKS 301 KGNGLSIEQW LSYGSEESLA FYAYREPKAA KQLHIGVIPK AVDEYLQMRG SYPAQEPDKK 361 LGNPVHHVHV ARGQDVPDAV LPVTFGLLLN LVAVMGADAA KDQIWRYLGQ YVAGADAATW 421 PELDRLIDNA ITYNRDYVAP TLKRRKPVGG EGAALKELDG RLAALPADAS ADDIQNIVYE 481 IGKNEAYGFE NLRDWFKALY ETLLGSSAGP RMGSFIALFG IDNTRRLIAE ALA //