LOCUS       AEG52710.1               885 aa    PRT              BCT 08-JUN-2015
DEFINITION  Sinorhizobium meliloti AK83 AMP-dependent synthetase and
            ligase protein.
ACCESSION   CP002781-935
PROTEIN_ID  AEG52710.1
SOURCE      Sinorhizobium meliloti AK83 (Ensifer meliloti AK83)
  ORGANISM  Sinorhizobium meliloti AK83
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
REFERENCE   1  (bases 1 to 3820344)
  AUTHORS   Galardini,M., Pini,F., Bazzicalupo,M., Biondi,E.G. and Mengoni,A.
  TITLE     Replicon-dependent bacterial genome evolution: the case of
            Sinorhizobium meliloti
  JOURNAL   Genome Biol Evol 5 (3), 542-558 (2013)
   PUBMED   23431003
REFERENCE   2  (bases 1 to 3820344)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Goodwin,L.,
            Pitluck,S., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M.,
            Hauser,L., Kyrpides,N., Ivanova,N., Ovchinnikova,G., Pagani,I.,
            Brilli,M., Bazzicalupo,M., Mengoni,A., Biondi,E.G. and Woyke,T.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Complete sequence of chromosome1 of Sinorhizobium meliloti AK83
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 3820344)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Goodwin,L.,
            Pitluck,S., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M.,
            Hauser,L., Kyrpides,N., Ivanova,N., Ovchinnikova,G., Pagani,I.,
            Brilli,M., Bazzicalupo,M., Mengoni,A., Biondi,E.G. and Woyke,T.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2011) US DOE Joint Genome Institute, 2800
            Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA
COMMENT     URL -- http://www.jgi.doe.gov
            JGI Project ID: 4086303
            Source DNA and organism available from Emanuele G. Biondi
            (emanuele.biondi@unifi.it)
            Contacts: Emanuele G. Biondi (emanuele.biondi@unifi.it)
                      Tanja Woyke (microbe@cuba.jgi-psf.org)
            Annotation done by JGI-ORNL and JGI-PGF
            Finishing done by JGI-LANL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. it is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            
            ##MIGS-Data-START##
            investigation_type       :: bacteria_archaea
            project_name             :: Sinorhizobium meliloti AK83
            collection_date          :: Missing
            lat_lon                  :: Missing
            depth                    :: Missing
            alt_elev                 :: Missing
            country                  :: Missing
            environment              :: Host, Plant root, Root nodule, Soil
            num_replicons            :: 5
            ref_biomaterial          :: Missing
            biotic_relationship      :: Symbiotic
            trophic_level            :: Missing
            rel_to_oxygen            :: Aerobe
            isol_growth_condt        :: Missing
            sequencing_meth          :: WGS
            assembly                 :: Newbler v. 2.3 (pre-release)
            finishing_strategy       :: Finished
            GOLD Stamp ID            :: Gi05780
            Current Finishing Status :: Finished
            Funding Program          :: DOE-CSP 2009
            Source of Isolate        :: Biondi, Emanuele (Universita di
                                        Firenze)
            Cell Shape               :: Rod-shaped
            Motility                 :: Motile
            Sporulation              :: Nonsporulating
            Temperature Range        :: Mesophile
            Temperature Optimum      :: 25 - 30C
            Gram Staining            :: Gram-
            Symbiotic Relationship   :: Mutualistic
            Diseases                 :: None
            Phenotypes               :: Nitrogen cycle, Streptomycin resistant,
                                        Symbiotic
            ##MIGS-Data-END##
            
            ##Genome-Assembly-Data-START##
            Finishing Goal           :: Finished
            Current Finishing Status :: Finished
            Assembly Method          :: Newbler v. 2.3
            Genome Coverage          :: 30x
            Sequencing Technology    :: 454/Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Qualifiers
     source          /organism="Sinorhizobium meliloti AK83"
                     /mol_type="genomic DNA"
                     /strain="AK83"
                     /db_xref="taxon:693982"
                     /chromosome="1"
                     /collected_by="Emmanuele Biondi"
     protein         /locus_tag="Sinme_0958"
                     /inference="protein motif:PFAM:PF00501"
                     /note="PFAM: AMP-dependent synthetase/ligase; KEGG:
                     smd:Smed_0772 AMP-dependent synthetase and ligase"
                     /transl_table=11
                     /db_xref="GO:0003824"
                     /db_xref="InterPro:IPR000873"
BEGIN
        1 MGGSGELSVS PFGVASLAAR GDRPALLLRG GRIVSYRELA ERIDRLASRW RGDRGLVTIE
       61 ADLSEHAVVA YLAALEAGHA VALCGTASML RDREVFRPEY CYRRFDGRWR MERLEGDAIA
      121 PHPELAVLLS TSGSTGHGKW VRLSHMNIQS NARSIAEYLG LTAADRGCLI LPLHYSYGLS
      181 VLNAHLAVGA SVYMPGCSIL DEGFLDELAQ SASSNFAGVP YSYDLLEKAG FRARDFPALR
      241 FMTVAGGRLA PELVRRYNEH LSARNARLFV MYGQTEATAR MAYMPPDRLR GREDRIGIAI
      301 PGGSLTIEDN DGRIISSADQ PGELVYRGPN VMMGYASSRA DLARGAELSE LRTGDLAVRD
      361 ADGVFRIVGR TKRISKIAGL RIGHDALEAA LEQHGVAAAV VGNDTEIHAY YVGSGDPDEV
      421 RRLLVEASGL TLMHVGASRL DKLPRLTSGK IDYPALRMRP AAATCGSSEE GDIEALFAQL
      481 FYPKKVRPED SFLSLGGDSL RFVQLSIGLE KRLGELPEGW EKMPVGALAA HNGRERKSRR
      541 IGADFLIRAL AILLVVVHHE TLWPIPGGSA AMVILAGFGL ARFQSGALLS GAIDKLLRPL
      601 AQILVPYYLI VAGYALAWGD VPWASVFLVG NFGIADPGLH GMVPYLYWFI EAYCQMLLVF
      661 GALFAVPFVR RAAAKRPLAV GMALFAAAFA ARMAFPLLID IGNRQIFTLP WIFYMAVLGW
      721 CAAIAETWKE RSMVMLAGTG VFLFLGLYEG VWIGTKVKYL LQIAVLAALL FLPRIRMPQW
      781 GRRLILPLSA AGFHIYILHR FVPELLLLPI RPLLSPFAFA LCSILGGIAL GIAAWLGQRR
      841 LIAWLAGTRD RRISLAPVGR LEPGLASVGP LLALGPQEGN QIQRQ
//