LOCUS AEG52710.1 885 aa PRT BCT 08-JUN-2015 DEFINITION Sinorhizobium meliloti AK83 AMP-dependent synthetase and ligase protein. ACCESSION CP002781-935 PROTEIN_ID AEG52710.1 SOURCE Sinorhizobium meliloti AK83 (Ensifer meliloti AK83) ORGANISM Sinorhizobium meliloti AK83 Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium. REFERENCE 1 (bases 1 to 3820344) AUTHORS Galardini,M., Pini,F., Bazzicalupo,M., Biondi,E.G. and Mengoni,A. TITLE Replicon-dependent bacterial genome evolution: the case of Sinorhizobium meliloti JOURNAL Genome Biol Evol 5 (3), 542-558 (2013) PUBMED 23431003 REFERENCE 2 (bases 1 to 3820344) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Ovchinnikova,G., Pagani,I., Brilli,M., Bazzicalupo,M., Mengoni,A., Biondi,E.G. and Woyke,T. CONSRTM US DOE Joint Genome Institute TITLE Complete sequence of chromosome1 of Sinorhizobium meliloti AK83 JOURNAL Unpublished REFERENCE 3 (bases 1 to 3820344) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Ovchinnikova,G., Pagani,I., Brilli,M., Bazzicalupo,M., Mengoni,A., Biondi,E.G. and Woyke,T. CONSRTM US DOE Joint Genome Institute TITLE Direct Submission JOURNAL Submitted (16-MAY-2011) US DOE Joint Genome Institute, 2800 Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA COMMENT URL -- http://www.jgi.doe.gov JGI Project ID: 4086303 Source DNA and organism available from Emanuele G. Biondi (emanuele.biondi@unifi.it) Contacts: Emanuele G. Biondi (emanuele.biondi@unifi.it) Tanja Woyke (microbe@cuba.jgi-psf.org) Annotation done by JGI-ORNL and JGI-PGF Finishing done by JGI-LANL The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. it is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). ##MIGS-Data-START## investigation_type :: bacteria_archaea project_name :: Sinorhizobium meliloti AK83 collection_date :: Missing lat_lon :: Missing depth :: Missing alt_elev :: Missing country :: Missing environment :: Host, Plant root, Root nodule, Soil num_replicons :: 5 ref_biomaterial :: Missing biotic_relationship :: Symbiotic trophic_level :: Missing rel_to_oxygen :: Aerobe isol_growth_condt :: Missing sequencing_meth :: WGS assembly :: Newbler v. 2.3 (pre-release) finishing_strategy :: Finished GOLD Stamp ID :: Gi05780 Current Finishing Status :: Finished Funding Program :: DOE-CSP 2009 Source of Isolate :: Biondi, Emanuele (Universita di Firenze) Cell Shape :: Rod-shaped Motility :: Motile Sporulation :: Nonsporulating Temperature Range :: Mesophile Temperature Optimum :: 25 - 30C Gram Staining :: Gram- Symbiotic Relationship :: Mutualistic Diseases :: None Phenotypes :: Nitrogen cycle, Streptomycin resistant, Symbiotic ##MIGS-Data-END## ##Genome-Assembly-Data-START## Finishing Goal :: Finished Current Finishing Status :: Finished Assembly Method :: Newbler v. 2.3 Genome Coverage :: 30x Sequencing Technology :: 454/Illumina ##Genome-Assembly-Data-END## FEATURES Qualifiers source /organism="Sinorhizobium meliloti AK83" /mol_type="genomic DNA" /strain="AK83" /db_xref="taxon:693982" /chromosome="1" /collected_by="Emmanuele Biondi" protein /locus_tag="Sinme_0958" /inference="protein motif:PFAM:PF00501" /note="PFAM: AMP-dependent synthetase/ligase; KEGG: smd:Smed_0772 AMP-dependent synthetase and ligase" /transl_table=11 /db_xref="GO:0003824" /db_xref="InterPro:IPR000873" BEGIN 1 MGGSGELSVS PFGVASLAAR GDRPALLLRG GRIVSYRELA ERIDRLASRW RGDRGLVTIE 61 ADLSEHAVVA YLAALEAGHA VALCGTASML RDREVFRPEY CYRRFDGRWR MERLEGDAIA 121 PHPELAVLLS TSGSTGHGKW VRLSHMNIQS NARSIAEYLG LTAADRGCLI LPLHYSYGLS 181 VLNAHLAVGA SVYMPGCSIL DEGFLDELAQ SASSNFAGVP YSYDLLEKAG FRARDFPALR 241 FMTVAGGRLA PELVRRYNEH LSARNARLFV MYGQTEATAR MAYMPPDRLR GREDRIGIAI 301 PGGSLTIEDN DGRIISSADQ PGELVYRGPN VMMGYASSRA DLARGAELSE LRTGDLAVRD 361 ADGVFRIVGR TKRISKIAGL RIGHDALEAA LEQHGVAAAV VGNDTEIHAY YVGSGDPDEV 421 RRLLVEASGL TLMHVGASRL DKLPRLTSGK IDYPALRMRP AAATCGSSEE GDIEALFAQL 481 FYPKKVRPED SFLSLGGDSL RFVQLSIGLE KRLGELPEGW EKMPVGALAA HNGRERKSRR 541 IGADFLIRAL AILLVVVHHE TLWPIPGGSA AMVILAGFGL ARFQSGALLS GAIDKLLRPL 601 AQILVPYYLI VAGYALAWGD VPWASVFLVG NFGIADPGLH GMVPYLYWFI EAYCQMLLVF 661 GALFAVPFVR RAAAKRPLAV GMALFAAAFA ARMAFPLLID IGNRQIFTLP WIFYMAVLGW 721 CAAIAETWKE RSMVMLAGTG VFLFLGLYEG VWIGTKVKYL LQIAVLAALL FLPRIRMPQW 781 GRRLILPLSA AGFHIYILHR FVPELLLLPI RPLLSPFAFA LCSILGGIAL GIAAWLGQRR 841 LIAWLAGTRD RRISLAPVGR LEPGLASVGP LLALGPQEGN QIQRQ //