LOCUS Y08418 1512 bp mRNA linear HUM 07-OCT-2008
DEFINITION Homo sapiens mRNA for nicotinic acetylcholine receptor alpha3
subunit precursor.
ACCESSION Y08418
VERSION Y08418.1
KEYWORDS nAChR gene; nicotinic acetylcholine receptor alpha 3 subunit.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1
AUTHORS Groot Kormelink P.J., Luyten W.H.M.L.
TITLE Cloning and sequence of full-length cDNAs encoding the human
neuronal nicotinic acetylcholine receptor (nAChR) subunits beta3
and beta4 and expression of seven nAChR subunits in the human
neuroblastoma cell line SH-SY5Y and/or IMR-32
JOURNAL FEBS Lett. 400(3), 309-314(1997).
PUBMED 9009220
REFERENCE 2 (bases 1 to 1512)
AUTHORS Groot Kormelink P.J.
JOURNAL Submitted (27-SEP-1996) to the INSDC. P.J. Groot Kormelink, Janssen
Research Foundation, Exp. Mol. Biol. Dept., Turnhoutseweg 30,
B-2340 Beerse, BELGIUM
REFERENCE 3
AUTHORS Groot-Kormelink P.J., Luyten W.H.M.L., Colquhoun D., Sivilotti L.G.
TITLE A reporter mutation approach shows incorporation of the orphan
subunit beta3 into a functional nicotinic receptor
JOURNAL J Biol Chem 273(25), 15317-15320(1998).
PUBMED 9624109
REFERENCE 4
AUTHORS Boorman J.P., Groot-Kormelink P.J., Sivilotti L.G.
TITLE Stoichiometry of human recombinant neuronal nicotinic receptors
containing the beta3 subunit expressed in Xenopus oocytes
JOURNAL J. Physiol. (Lond.) 529, 565-577(2000).
REFERENCE 5
AUTHORS Groot-Kormelink P.J., Boorman J.P., Sivilotti L.G.
TITLE Formation of functional alpha3beta4alpha5 human neuronal nicotinic
receptors in Xenopus oocytes: a reporter mutation approach
JOURNAL Br. J. Pharmacol. 134(4), 789-796(2001).
PUBMED 11606319
REFERENCE 6
AUTHORS Boorman J.P., Beato M., Groot-Kormelink P.J., Broadbent S.D.,
Sivilotti L.G.
TITLE The effects of beta3 subunit incorporation on the pharmacology and
single channel properties of oocyte-expressed human alpha3beta4
neuronal nicotinic receptors
JOURNAL J Biol Chem 278(45), 44033-44040(2003).
PUBMED 12912995
FEATURES Location/Qualifiers
source 1..1512
/db_xref="H-InvDB:HIT000325449"
/organism="Homo sapiens"
/mol_type="mRNA"
/cell_line="IMR-32"
/cell_line="SH-SY5Y"
/cell_type="neuroblastoma"
/db_xref="taxon:9606"
sig_peptide 1..87
CDS 1..1512
/gene="nAChRA3"
/product="nicotinic acetylcholine receptor alpha3 subunit
precursor"
/db_xref="GOA:P32297"
/db_xref="H-InvDB:HIT000325449.12"
/db_xref="HGNC:HGNC:1957"
/db_xref="InterPro:IPR002394"
/db_xref="InterPro:IPR006029"
/db_xref="InterPro:IPR006201"
/db_xref="InterPro:IPR006202"
/db_xref="InterPro:IPR018000"
/db_xref="InterPro:IPR036719"
/db_xref="InterPro:IPR036734"
/db_xref="PDB:4ZK4"
/db_xref="PDB:5SYO"
/db_xref="PDB:5TVC"
/db_xref="UniProtKB/Swiss-Prot:P32297"
/protein_id="CAA69695.1"
/translation="MALAVSLPLALSPPRLLLLLLSLLPVARASEAEHRLFERLFEDY
NEIIRPVANVSDPVIIHFEVSMSQLVKVDEVNQIMETNLWLKQIWNDYKLKWNPSDYG
GAEFMRVPAQKIWKPDIVLYNNAVGDFQVDDKTKALLKYTGEVTWIPPAIFKSSCKID
VTYFPFDYQNCTMKFGSWSYDKAKIDLVLIGSSMNLKDYWESGEWAIIKAPGYKHDIK
YNCCEEIYPDITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCIS
VLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPTTH
TMPSWVKTVFLNLLPRVMFMTRPTSNEGNAQKPRPLYGAELSNLNCFSRAESKGCKEG
YPCQDGMCGYCHHRRIKISNFSANLTRSSSSESVDAVLSLSALSPEIKEAIQSVKYIA
ENMKAQNEAKEIQDDWKYVAMVIDRIFLWVFTLVCILGTAGLFLQPLMAREDA"
mat_peptide 88..1509
/gene="nAChRA3"
/product="nicotinic acetylcholine receptor alpha3 subunit"
BASE COUNT 352 a 424 c 364 g 372 t
ORIGIN
1 atggctctgg ccgtctcgct gcccctggcg ctgtcgccgc cgcggctgct gctgctgctg
61 ctgtctctgc tgccagtggc cagggcctca gaggctgagc accgtctatt tgagcggctg
121 tttgaagatt acaatgagat catccggcct gtagccaacg tgtctgaccc agtcatcatc
181 catttcgagg tgtccatgtc tcagctggtg aaggtggatg aagtaaacca gatcatggag
241 accaacctgt ggctcaagca aatctggaat gactacaagc tgaagtggaa cccctctgac
301 tatggtgggg cagagttcat gcgtgtccct gcacagaaga tctggaagcc agacattgtg
361 ctgtataaca atgctgttgg ggatttccag gtggacgaca agaccaaagc cttactcaag
421 tacactgggg aggtgacttg gatacctccg gccatcttta agagctcctg taaaatcgac
481 gtgacctact tcccgtttga ttaccaaaac tgtaccatga agttcggttc ctggtcctac
541 gataaggcga aaatcgatct ggtcctgatc ggctcttcca tgaacctcaa ggactattgg
601 gagagcggcg agtgggccat catcaaagcc ccaggctata aacacgacat caagtacaac
661 tgctgcgagg agatctaccc cgacatcaca tactcgctgt acatccggcg cctgcccttg
721 ttctacacca tcaacctcat catcccctgc ctgctcatct ccttcctcac tgtgctcgtc
781 ttctacctgc cctccgactg cggtgagaag gtgaccctgt gcatttctgt cctcctctcc
841 ctgacggtgt ttctcctggt gatcactgag accatccctt ccacctcgct ggtcatcccc
901 ctgattggag agtacctcct gttcaccatg atttttgtaa ccttgtccat cgtcatcacc
961 gtcttcgtgc tcaacgtgca ctacagaacc ccgacgacac acacaatgcc ctcatgggtg
1021 aagactgtat tcttgaacct gctccccagg gtcatgttca tgaccaggcc aacaagcaac
1081 gagggcaacg ctcagaagcc gaggcccctc tacggtgccg agctctcaaa tctgaattgc
1141 ttcagccgcg cagagtccaa aggctgcaag gagggctacc cctgccagga cgggatgtgt
1201 ggttactgcc accaccgcag gataaaaatc tccaatttca gtgctaacct cacgagaagc
1261 tctagttctg aatctgttga tgctgtgctg tccctctctg ctttgtcacc agaaatcaaa
1321 gaagccatcc aaagtgtcaa gtatattgct gaaaatatga aagcacaaaa tgaagccaaa
1381 gagattcaag atgattggaa gtatgttgcc atggtgattg atcgtatttt tctgtgggtt
1441 ttcaccctgg tgtgcattct agggacagca ggattgtttc tgcaacccct gatggccagg
1501 gaagatgcat aa
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