LOCUS HUMMDR1 4646 bp mRNA linear HUM 03-DEC-1999
DEFINITION Homo sapiens P-glycoprotein (PGY1) mRNA, complete cds.
ACCESSION M14758
VERSION M14758.1
KEYWORDS P-glycoprotein; drug resistance protein; transport protein.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4646)
AUTHORS Chen,C.J., Chin,J.E., Ueda,K., Clark,D.P., Pastan,I.,
Gottesman,M.M. and Roninson,I.B.
TITLE Internal duplication and homology with bacterial transport proteins
in the mdr1 (P-glycoprotein) gene from multidrug-resistant human
cells
JOURNAL Cell 47 (3), 381-389 (1986)
PUBMED 2876781
REFERENCE 2 (sites)
AUTHORS Ueda,K., Clark,D.P., Chen,C.J., Roninson,I.B., Gottesman,M.M. and
Pastan,I.
TITLE The human multidrug resistance (mdr1) gene. cDNA cloning and
transcription initiation
JOURNAL J. Biol. Chem. 262 (2), 505-508 (1987)
PUBMED 3027054
REFERENCE 3 (bases 971 to 985; 3095 to 3109)
AUTHORS Kioka,N., Tsubota,J., Kakehi,Y., Komano,T., Gottesman,M.M.,
Pastan,I. and Ueda,K.
TITLE P-glycoprotein gene (MDR1) cDNA from human adrenal: normal
P-glycoprotein carries Gly185 with an altered pattern of multidrug
resistance
JOURNAL Biochem. Biophys. Res. Commun. 162 (1), 224-231 (1989)
PUBMED 2568832
COMMENT Original source text: Human drug-resistant cell line KB-C2.5 cDNA
to mRNA, clones lambda-HDR[10, 5, 104].
[2] sites.
Draft entry and computer-readable sequence [1] kindly submitted by
I.B.Roninson, 13-AUG-1987.
The sequence shown is of a cDNA clone initiating at a minor
upstream transcription initiation site and containing the major
site of transcription initiation.
FEATURES Location/Qualifiers
source 1..4646
/db_xref="H-InvDB:HIT000194362"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/map="7q21"
gene 1..4646
/gene="PGY1"
variation 382
/gene="PGY1"
/note="g in [1]; a in [2]"
CDS 425..4267
/gene="PGY1"
/note="P-glycoprotein"
/codon_start=1
/protein_id="AAA59575.1"
/translation="MDLEGDRNGGAKKKNFFKLNNKSEKDKKEKKPTVSVFSMFRYSN
WLDKLYMVVGTLAAIIHGAGLPLMMLVFGEMTDIFANAGNLEDLMSNITNRSDINDTG
FFMNLEEDMTRYAYYYSGIGAGVLVAAYIQVSFWCLAAGRQIHKIRKQFFHAIMRQEI
GWFDVHDVGELNTRLTDDVSKINEVIGDKIGMFFQSMATFFTGFIVGFTRGWKLTLVI
LAISPVLGLSAAVWAKILSSFTDKELLAYAKAGAVAEEVLAAIRTVIAFGGQKKELER
YNKNLEEAKRIGIKKAITANISIGAAFLLIYASYALAFWYGTTLVLSGEYSIGQVLTV
FFSVLIGAFSVGQASPSIEAFANARGAAYEIFKIIDNKPSIDSYSKSGHKPDNIKGNL
EFRNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTEGM
VSVDGQDIRTINVRFLREIIGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEAN
AYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESE
AVVQVALDKARKGRTTIVIAHRLSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFK
LVTMQTAGNEVELENAADESKSEIDALEMSSNDSRSSLIRKRSTRRSVRGSQAQDRKL
STKEALDESIPPVSFWRIMKLNLTEWPYFVVGVFCAIINGGLQPAFAIIFSKIIGVFT
RIDDPETKRQNSNLFSLLFLALGIISFITFFLQGFTFGKAGEILTKRLRYMVFRSMLR
QDVSWFDDPKNTTGALTTRLANDAAQVKGAIGSRLAVITQNIANLGTGIIISFIYGWQ
LTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGAGKIATEAIENFRTVVSLTQEQ
KFEHMYAQSLQVPYRNSLRKAHIFGITFSFTQAMMYFSYAGCFRFGAYLVAHKLMSFE
DVLLVFSAVVFGAMAVGQVSSFAPDYAKAKISAAHIIMIIEKTPLIDSYSTEGLMPNT
LEGNVTFGEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYD
PLAGKVLLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQEEIV
RAAKEANIHAFIESLPNKYSTKVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATS
ALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGRVKEHGTHQQLLA
QKGIYFSMVSVQAGTKRQ"
variation 964
/gene="PGY1"
/note="t in [1]; c in [2]"
variation 978..979
/gene="PGY1"
/note="tt in [1]; ga in [2] Val->Gly"
variation 1660
/gene="PGY1"
/note="c in [1]; t in [2]"
variation 2065
/gene="PGY1"
/note="c in [1]; t in [2]"
variation 3101
/gene="PGY1"
/note="g in [1]; t in [2] Ala-> Ser"
variation 3859
/gene="PGY1"
/note="c in [1]; t in [2]"
variation 4460
/gene="PGY1"
/note="a in [1]; g in [2]"
BASE COUNT 1371 a 892 c 1129 g 1254 t
ORIGIN 154 bp upstream of AvaI site; chromosome 7q21.1.
1 cctactctat tcagatattc tccagattcc taaagattag agatcatttc tcattctcct
61 aggagtactc acttcaggaa gcaaccagat aaaagagagg tgcaacggaa gccagaacat
121 tcctcctgga aattcaacct gtttcgcagt ttctcgagga atcagcattc agtcaatccg
181 ggccgggagc agtcatctgt ggtgaggctg attggctggg caggaacagc gccggggcgt
241 gggctgagca cagcgcttcg ctctctttgc cacaggaagc ctgagctcat tcgagtagcg
301 gctcttccaa gctcaaagaa gcagaggccg ctgttcgttt cctttaggtc tttccactaa
361 agtcggagta tcttcttcca agatttcacg tcttggtggc cgttccaagg agcgcgaggt
421 cgggatggat cttgaagggg accgcaatgg aggagcaaag aagaagaact tttttaaact
481 gaacaataaa agtgaaaaag ataagaagga aaagaaacca actgtcagtg tattttcaat
541 gtttcgctat tcaaattggc ttgacaagtt gtatatggtg gtgggaactt tggctgccat
601 catccatggg gctggacttc ctctcatgat gctggtgttt ggagaaatga cagatatctt
661 tgcaaatgca ggaaatttag aagatctgat gtcaaacatc actaatagaa gtgatatcaa
721 tgatacaggg ttcttcatga atctggagga agacatgacc aggtatgcct attattacag
781 tggaattggt gctggggtgc tggttgctgc ttacattcag gtttcatttt ggtgcctggc
841 agctggaaga caaatacaca aaattagaaa acagtttttt catgctataa tgcgacagga
901 gataggctgg tttgatgtgc acgatgttgg ggagcttaac acccgactta cagatgatgt
961 ctctaagatt aatgaagtta ttggtgacaa aattggaatg ttctttcagt caatggcaac
1021 atttttcact gggtttatag taggatttac acgtggttgg aagctaaccc ttgtgatttt
1081 ggccatcagt cctgttcttg gactgtcagc tgctgtctgg gcaaagatac tatcttcatt
1141 tactgataaa gaactcttag cgtatgcaaa agctggagca gtagctgaag aggtcttggc
1201 agcaattaga actgtgattg catttggagg acaaaagaaa gaacttgaaa ggtacaacaa
1261 aaatttagaa gaagctaaaa gaattgggat aaagaaagct attacagcca atatttctat
1321 aggtgctgct ttcctgctga tctatgcatc ttatgctctg gccttctggt atgggaccac
1381 cttggtcctc tcaggggaat attctattgg acaagtactc actgtattct tttctgtatt
1441 aattggggct tttagtgttg gacaggcatc tccaagcatt gaagcatttg caaatgcaag
1501 aggagcagct tatgaaatct tcaagataat tgataataag ccaagtattg acagctattc
1561 gaagagtggg cacaaaccag ataatattaa gggaaatttg gaattcagaa atgttcactt
1621 cagttaccca tctcgaaaag aagttaagat cttgaagggc ctgaacctga aggtgcagag
1681 tgggcagacg gtggccctgg ttggaaacag tggctgtggg aagagcacaa cagtccagct
1741 gatgcagagg ctctatgacc ccacagaggg gatggtcagt gttgatggac aggatattag
1801 gaccataaat gtaaggtttc tacgggaaat cattggtgtg gtgagtcagg aacctgtatt
1861 gtttgccacc acgatagctg aaaacattcg ctatggccgt gaaaatgtca ccatggatga
1921 gattgagaaa gctgtcaagg aagccaatgc ctatgacttt atcatgaaac tgcctcataa
1981 atttgacacc ctggttggag agagaggggc ccagttgagt ggtgggcaga agcagaggat
2041 cgccattgca cgtgccctgg ttcgcaaccc caagatcctc ctgctggatg aggccacgtc
2101 agccttggac acagaaagcg aagcagtggt tcaggtggct ctggataagg ccagaaaagg
2161 tcggaccacc attgtgatag ctcatcgttt gtctacagtt cgtaatgctg acgtcatcgc
2221 tggtttcgat gatggagtca ttgtggagaa aggaaatcat gatgaactca tgaaagagaa
2281 aggcatttac ttcaaacttg tcacaatgca gacagcagga aatgaagttg aattagaaaa
2341 tgcagctgat gaatccaaaa gtgaaattga tgccttggaa atgtcttcaa atgattcaag
2401 atccagtcta ataagaaaaa gatcaactcg taggagtgtc cgtggatcac aagcccaaga
2461 cagaaagctt agtaccaaag aggctctgga tgaaagtata cctccagttt ccttttggag
2521 gattatgaag ctaaatttaa ctgaatggcc ttattttgtt gttggtgtat tttgtgccat
2581 tataaatgga ggcctgcaac cagcatttgc aataatattt tcaaagatta taggggtttt
2641 tacaagaatt gatgatcctg aaacaaaacg acagaatagt aacttgtttt cactattgtt
2701 tctagccctt ggaattattt cttttattac atttttcctt cagggtttca catttggcaa
2761 agctggagag atcctcacca agcggctccg atacatggtt ttccgatcca tgctcagaca
2821 ggatgtgagt tggtttgatg accctaaaaa caccactgga gcattgacta ccaggctcgc
2881 caatgatgct gctcaagtta aaggggctat aggttccagg cttgctgtaa ttacccagaa
2941 tatagcaaat cttgggacag gaataattat atccttcatc tatggttggc aactaacact
3001 gttactctta gcaattgtac ccatcattgc aatagcagga gttgttgaaa tgaaaatgtt
3061 gtctggacaa gcactgaaag ataagaaaga actagaaggt gctgggaaga tcgctactga
3121 agcaatagaa aacttccgaa ccgttgtttc tttgactcag gagcagaagt ttgaacatat
3181 gtatgctcag agtttgcagg taccatacag aaactctttg aggaaagcac acatctttgg
3241 aattacattt tccttcaccc aggcaatgat gtatttttcc tatgctggat gtttccggtt
3301 tggagcctac ttggtggcac ataaactcat gagctttgag gatgttctgt tagtattttc
3361 agctgttgtc tttggtgcca tggccgtggg gcaagtcagt tcatttgctc ctgactatgc
3421 caaagccaaa atatcagcag cccacatcat catgatcatt gaaaaaaccc ctttgattga
3481 cagctacagc acggaaggcc taatgccgaa cacattggaa ggaaatgtca catttggtga
3541 agttgtattc aactatccca cccgaccgga catcccagtg cttcagggac tgagcctgga
3601 ggtgaagaag ggccagacgc tggctctggt gggcagcagt ggctgtggga agagcacagt
3661 ggtccagctc ctggagcggt tctacgaccc cttggcaggg aaagtgctgc ttgatggcaa
3721 agaaataaag cgactgaatg ttcagtggct ccgagcacac ctgggcatcg tgtcccagga
3781 gcccatcctg tttgactgca gcattgctga gaacattgcc tatggagaca acagccgggt
3841 ggtgtcacag gaagagatcg tgagggcagc aaaggaggcc aacatacatg ccttcatcga
3901 gtcactgcct aataaatata gcactaaagt aggagacaaa ggaactcagc tctctggtgg
3961 ccagaaacaa cgcattgcca tagctcgtgc ccttgttaga cagcctcata ttttgctttt
4021 ggatgaagcc acgtcagctc tggatacaga aagtgaaaag gttgtccaag aagccctgga
4081 caaagccaga gaaggccgca cctgcattgt gattgctcac cgcctgtcca ccatccagaa
4141 tgcagactta atagtggtgt ttcagaatgg cagagtcaag gagcatggca cgcatcagca
4201 gctgctggca cagaaaggca tctatttttc aatggtcagt gtccaggctg gaacaaagcg
4261 ccagtgaact ctgactgtat gagatgttaa atacttttta atatttgttt agatatgaca
4321 tttattcaaa gttaaaagca aacacttaca gaattatgaa gaggtatctg tttaacattt
4381 cctcagtcaa gttcagagtc ttcagagact tcgtaattaa aggaacagag tgagagacat
4441 catcaagtgg agagaaatca tagtttaaac tgcattataa attttataac agaattaaag
4501 tagattttaa aagataaaat gtgtaatttt gtttatattt tcccatttgg actgtaactg
4561 actgccttgc taaaagatta tagaagtagc aaaaagtatt gaaatgtttg cataaagtgt
4621 ctataataaa actaaacttt catgtg
//