LOCUS AF148213 4193 bp mRNA linear HUM 30-JUN-1999
DEFINITION Homo sapiens aggrecanase-1 mRNA, complete cds.
ACCESSION AF148213
VERSION AF148213.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4193)
AUTHORS Tortorella,M.D., Burn,T.C., Pratta,M.A., Abbaszade,I., Hollis,J.M.,
Liu,R., Rosenfeld,S.A., Copeland,R.A., Decicco,C.P., Wynn,R.,
Rockwell,A., Yang,F., Duke,J.L., Solomon,K., George,H.,
Bruckner,R., Nagase,H., Itoh,Y., Ellis,D.M., Ross,H., Wiswall,B.H.,
Murphy,K., Hillman,M.C. Jr., Hollis,G.F., Newton,R.C.,
Magolda,R.L., Trzaskos,J.M. and Arner,E.C.
TITLE Purification and cloning of aggrecanase-1: a member of the ADAMTS
family of proteins
JOURNAL Science 284 (5420), 1664-1666 (1999)
PUBMED 10356395
REFERENCE 2 (bases 1 to 4193)
AUTHORS Tortorella,M.D., Burn,T.C., Pratta,M.A., Abbaszade,I., Hollis,J.M.,
Liu,R.-Q., Rosenfeld,S.A., Copeland,R.A., Decicco,C.P., Wynn,R.,
Rockewell,A., Yang,F., Duke,J.L., Solomon,K., George,H.J.,
Bruchner,R., Nagase,H., Ito,Y., Ellis,D.M., Ross,O.H.,
Wiswall,B.H., Murphy,K., Hillman,M.C. Jr., Hollis,G.F.,
Newton,R.C., Magolda,R.L., Trzaskos,J.M. and Arner,E.C.
TITLE Direct Submission
JOURNAL Submitted (03-MAY-1999) Applied Biotechnology, DuPont
Pharmaceuticals Company, Experimental Station, E336/237B,
Wilmington, DE 19880, USA
FEATURES Location/Qualifiers
source 1..4193
/db_xref="H-InvDB:HIT000071532"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
CDS 407..2920
/note="aggrecan degrading metalloproteinase-1; disintegrin
metalloproteinase; ADMP-1; ADAMTS-4"
/codon_start=1
/product="aggrecanase-1"
/protein_id="AAD41494.1"
/translation="MSQTGSHPGRGLAGRWLWGAQPCLLLPIVPLSWLVWLLLLLLAS
LLPSARLASPLPREEEIVFPEKLNGSVLPGSGAPARLLCRLQAFGETLLLELEQDSGV
QVEGLTVQYLGQAPELLGGAEPGTYLTGTINGDPESVASLHWDGGALLGVLQYRGAEL
HLQPLEGGTPNSAGGPGAHILRRKSPASGQGPMCNVKAPLGSPSPRPRRAKRFASLSR
FVETLVVADDKMAAFHGAGLKRYLLTVMAAAAKAFKHPSIRNPVSLVVTRLVILGSGE
EGPQVGPSAAQTLRSFCAWQRGLNTPEDSDPDHFDTAILFTRQDLCGVSTCDTLGMAD
VGTVCDPARSCAIVEDDGLQSAFTAAHELGHVFNMLHDNSKPCISLNGPLSTSRHVMA
PVMAHVDPEEPWSPCSARFITDFLDNGYGHCLLDKPEAPLHLPVTFPGKDYDADRQCQ
LTFGPDSRHCPQLPPPCAALWCSGHLNGHAMCQTKHSPWADGTPCGPAQACMGGRCLH
MDQLQDFNIPQAGGWGPWGPWGDCSRTCGGGVQFSSRDCTRPVPRNGGKYCEGRRTRF
RSCNTEDCPTGSALTFREEQCAAYNHRTDLFKSFPGPMDWVPRYTGVAPQDQCKLTCQ
ARALGYYYVLEPRVVDGTPCSPDSSSVCVQGRCIHAGCDRIIGSKKKFDKCMVCGGDG
SGCSKQSGSFRKFRYGYNNVVTIPAGATHILVRQQGNPGHRSIYLALKLPDGSYALNG
EYTLMPSPTDVVLPGAVSLRYSGATAASETLSGHGPLAQPLTLQVLVAGNPQDTRLRY
SFFVPRPTPSTPRPTPQDWLHRRAQILEILRRRPWAGRK"
BASE COUNT 807 a 1249 c 1220 g 917 t
ORIGIN
1 cacagacaca tatgcacgag agagacagag gaggaaagag acagagacaa aggcacagcg
61 gaagaaggca gagacagggc aggcacagaa gcggcccaga cagagtccta cagagggaga
121 ggccagagaa gctgcagaag acacaggcag ggagagacaa agatccagga aaggagggct
181 caggaggaga gtttggagaa gccagacccc tgggcacctc tcccaagccc aaggactaag
241 ttttctccat ttcctttaac ggtcctcagc ccttctgaaa actttgcctc tgaccttggc
301 aggagtccaa gcccccaggc tacagagagg agctttccaa agctagggtg tggaggactt
361 ggtgccctag acggcctcag tccctcccag ctgcagtacc agtgccatgt cccagacagg
421 ctcgcatccc gggaggggct tggcagggcg ctggctgtgg ggagcccaac cctgcctcct
481 gctccccatt gtgccgctct cctggctggt gtggctgctt ctgctactgc tggcctctct
541 cctgccctca gcccggctgg ccagccccct cccccgggag gaggagatcg tgtttccaga
601 gaagctcaac ggcagcgtcc tgcctggctc gggcgcccct gccaggctgt tgtgccgctt
661 gcaggccttt ggggagacgc tgctactaga gctggagcag gactccggtg tgcaggtcga
721 ggggctgaca gtgcagtacc tgggccaggc gcctgagctg ctgggtggag cagagcctgg
781 cacctacctg actggcacca tcaatggaga tccggagtcg gtggcatctc tgcactggga
841 tgggggagcc ctgttaggcg tgttacaata tcggggggct gaactccacc tccagcccct
901 ggagggaggc acccctaact ctgctggggg acctggggct cacatcctac gccggaagag
961 tcctgccagc ggtcaaggtc ccatgtgcaa cgtcaaggct cctcttggaa gccccagccc
1021 cagaccccga agagccaagc gctttgcttc actgagtaga tttgtggaga cactggtggt
1081 ggcagatgac aagatggccg cattccacgg tgcggggcta aagcgctacc tgctaacagt
1141 gatggcagca gcagccaagg ccttcaagca cccaagcatc cgcaatcctg tcagcttggt
1201 ggtgactcgg ctagtgatcc tggggtcagg cgaggagggg ccccaagtgg ggcccagtgc
1261 tgcccagacc ctgcgcagct tctgtgcctg gcagcggggc ctcaacaccc ctgaggactc
1321 ggaccctgac cactttgaca cagccattct gtttacccgt caggacctgt gtggagtctc
1381 cacttgcgac acgctgggta tggctgatgt gggcaccgtc tgtgacccgg ctcggagctg
1441 tgccattgtg gaggatgatg ggctccagtc agccttcact gctgctcatg aactgggtca
1501 tgtcttcaac atgctccatg acaactccaa gccatgcatc agtttgaatg ggcctttgag
1561 cacctctcgc catgtcatgg cccctgtgat ggctcatgtg gatcctgagg agccctggtc
1621 cccctgcagt gcccgcttca tcactgactt cctggacaat ggctatgggc actgtctctt
1681 agacaaacca gaggctccat tgcatctgcc tgtgactttc cctggcaagg actatgatgc
1741 tgaccgccag tgccagctga ccttcgggcc cgactcacgc cattgtccac agctgccgcc
1801 gccctgtgct gccctctggt gctctggcca cctcaatggc catgccatgt gccagaccaa
1861 acactcgccc tgggccgatg gcacaccctg cgggcccgca caggcctgca tgggtggtcg
1921 ctgcctccac atggaccagc tccaggactt caatattcca caggctggtg gctggggtcc
1981 ttggggacca tggggtgact gctctcggac ctgtgggggt ggtgtccagt tctcctcccg
2041 agactgcacg aggcctgtcc cccggaatgg tggcaagtac tgtgagggcc gccgtacccg
2101 cttccgctcc tgcaacactg aggactgccc aactggctca gccctgacct tccgcgagga
2161 gcagtgtgct gcctacaacc accgcaccga cctcttcaag agcttcccag ggcccatgga
2221 ctgggttcct cgctacacag gcgtggcccc ccaggaccag tgcaaactca cctgccaggc
2281 ccgggcactg ggctactact atgtgctgga gccacgggtg gtagatggga ccccctgttc
2341 cccggacagc tcctcggtct gtgtccaggg ccgatgcatc catgctggct gtgatcgcat
2401 cattggctcc aagaagaagt ttgacaagtg catggtgtgc ggaggggacg gttctggttg
2461 cagcaagcag tcaggctcct tcaggaaatt caggtacgga tacaacaatg tggtcactat
2521 ccccgcgggg gccacccaca ttcttgtccg gcagcaggga aaccctggcc accggagcat
2581 ctacttggcc ctgaagctgc cagatggctc ctatgccctc aatggtgaat acacgctgat
2641 gccctccccc acagatgtgg tactgcctgg ggcagtcagc ttgcgctaca gcggggccac
2701 tgcagcctca gagacactgt caggccatgg gccactggcc cagcctttga cactgcaagt
2761 cctagtggct ggcaaccccc aggacacacg cctccgatac agcttcttcg tgccccggcc
2821 gaccccttca acgccacgcc ccactcccca ggactggctg caccgaagag cacagattct
2881 ggagatcctt cggcggcgcc cctgggcggg caggaaataa cctcactatc ccggctgccc
2941 tttctgggca ccggggcctc ggacttagct gggagaaaga gagagcttct gttgctgcct
3001 catgctaaga ctcagtgggg aggggctgtg ggcgtgagac ctgcccctcc tctctgccct
3061 aatgcgcagg ctggccctgc cctggtttcc tgccctggga ggcagtgatg ggttagtgga
3121 tggaaggggc tgacagacag ccctccatct aaactgcccc ctctgccctg cgggtcacag
3181 gagggagggg gaaggcaggg agggcctggg ccccagttgt atttatttag tatttattca
3241 cttttattta gcaccaggga aggggacaag gactagggtc ctggggaacc tgacccctga
3301 cccctcatag ccctcaccct ggggctagga aatccagggt ggtggtgata ggtataagtg
3361 gtgtgtgtat gcgtgtgtgt gtgtgtgtga aaatgtgtgt gtgcttatgt atgaggtaca
3421 acctgttctg ctttcctctt cctgaatttt attttttggg aaaagaaaag tcaagggtag
3481 ggtgggcctt cagggagtga gggattatcc tttttttttt ctttctttct ttcttttttt
3541 ttttgagaca gaatctcgct ctgtcgccca ggctggagtg caatggcaca atctcggctc
3601 actgcatcct ccgcctcccg ggttcaagtg attctcatgc ctcagcctcc tgagtagctg
3661 ggattacagg ctcctgccac cacgcccggc taatttttgt tttgttttgt ttggagacag
3721 agtctcgcta ttgtcaccag ggctggaatg atttcagctc actgcaacct tcgccacctg
3781 ggttccagca attctcctgc ctcagcctcc cgagtagctg agattatagg cacctaccac
3841 cacgcccggc taatttttgt atttttagta gagacggggt ttcaccatgt tggccaggct
3901 ggtctcgaac tcctgacctt aggtgatcca ctcgccttca tctcccaaag tgctgggatt
3961 acaggcgtga gccaccgtgc ctggccacgc ccaactaatt tttgtatttt tagtagagac
4021 agggtttcac catgttggcc aggctgctct tgaactcctg acctcaggta atcgacctgc
4081 ctcggcctcc caaagtgctg ggattacagg tgtgagccac cacgcccggt acatattttt
4141 taaattgaat tctactattt atgtgatcct tttggagtca gacagatgtg ggt
//