LOCUS CVU30497 8392 bp DNA circular SYN 09-APR-1996 DEFINITION Cloning vector pAS2-1, complete sequence. ACCESSION U30497 VERSION U30497.1 KEYWORDS . SOURCE Cloning vector pAS2-1 ORGANISM Cloning vector pAS2-1 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 8392) AUTHORS Kitts,P.A. TITLE CLONTECH Vectors On Disk, version 1.3 JOURNAL Unpublished REFERENCE 2 (bases 1 to 8392) AUTHORS Stile,J.S. TITLE Direct Submission JOURNAL Submitted (28-JUN-1995) John S. Stile, CLONTECH Laboratories, Inc., 4030 Fabian Way, Palo Alto, CA 94303, USA COMMENT On Apr 9, 1996 this sequence version replaced gi:988208. This vector can be obtained from CLONTECH Laboratories, Inc., 4030 Fabian Way, Palo Alto, CA 94303, USA. To place an order call (415) 424-8222 or (800) 662-2566, extension 1. International customers, please contact your local distributor. For technical information, call (415) 424-8222 or (800) 662-2566, extension 3. This sequence has been compiled from information in the sequence databases, published literature and other sources, together with partial sequences obtained by CLONTECH. If you suspect there is an error in this sequence, please contact CLONTECH's Technical Service Department at (415) 424-8222 or (800) 662-2566, extension 3 or E-mail TECH@CLONTECH.COM. FEATURES Location/Qualifiers source 1..8392 /organism="Cloning vector pAS2-1" /mol_type="genomic DNA" /db_xref="taxon:42098" rep_origin 1..1348 /note="two micron origin of replication (B form)" gene 1884..2558 /gene="TRP1" CDS 1884..2558 /gene="TRP1" /codon_start=1 /transl_table=11 /protein_id="AAA96381.1" /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF VKNAKK" rep_origin 2609..3277 /note="f1+ origin" gene complement(3800..4304) /gene="CYH2" CDS complement(3855..4304) /gene="CYH2" /codon_start=1 /transl_table=11 /protein_id="AAA96382.1" /translation="MPSRFTKTRKHRGHVSAGKGRIGKHRKHPGGRGMAGGQHHHRIN MDKYHPGYFGKVGMRYFHKQQAHFWKPVLNLDKLWTLIPEDKRDQYLKSASKETAPVI DTLAAGYGKILGKGRIPNVPVIVKARFVSKLAEEKIRAAGGVVELIA" regulatory 4767..5474 /regulatory_class="promoter" /note="from ADH1 gene" CDS 5501..6064 /note="contains Gal4 binding domain and epitopes for monoclonal antibody binding" /codon_start=1 /transl_table=11 /product="fusion protein" /protein_id="AAA96383.1" /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPVLQYPALTHMAM EAEFPGIRRPAAKLIPGEFLMIYDFYY" misc_feature 5501..5941 /note="encodes Gal4 binding domain" misc_feature 5952..5969 /note="encodes epitope for monoclonals D11 and F10 binding" misc_feature 5970..6015 /note="multiple cloning site" regulatory 6031..6223 /regulatory_class="terminator" /note="from ADH1 gene; contains stop codons for frame 1,2 and 3" rep_origin 6231..7402 /note="pUC origin of replication" gene complement(7402..8262) /gene="ampicillin resistance" CDS complement(7402..8262) /gene="ampicillin resistance" /codon_start=1 /transl_table=11 /protein_id="AAA96384.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 2351 a 1754 c 1870 g 2417 t ORIGIN 1 gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt 61 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt 121 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta 181 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg 241 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag 301 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat 361 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg 421 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact 481 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga 541 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca 601 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac 661 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa 721 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag 781 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag 841 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt 901 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg 961 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt 1021 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca 1081 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt 1141 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc 1201 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact 1261 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc 1321 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta 1381 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 1441 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 1501 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 1561 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat 1621 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac 1681 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca 1741 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa 1801 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta 1861 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat 1921 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg 1981 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg 2041 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact 2101 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg 2161 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg 2221 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag 2281 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg 2341 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct 2401 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa 2461 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta 2521 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat 2581 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa 2641 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa 2701 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 2761 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 2821 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 2881 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 2941 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 3001 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 3061 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg 3121 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 3181 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 3241 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga 3301 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt 3361 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata 3421 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag 3481 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc 3541 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt 3601 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca 3661 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt 3721 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga 3781 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct 3841 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct 3901 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg gattctaccc 3961 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta 4021 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc 4081 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc 4141 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg 4201 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag 4261 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat 4321 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag 4381 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata 4441 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg 4501 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat 4561 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt 4621 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc 4681 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca 4741 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg 4801 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt 4861 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca 4921 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa 4981 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt 5041 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt 5101 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac 5161 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga 5221 tgaggggtat cttcgaacac acgaaacttt ttccttcctt cattcacgca cactactctc 5281 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt 5341 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat 5401 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag 5461 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat 5521 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa 5581 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc 5641 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct 5701 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca 5761 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt 5821 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat 5881 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc 5941 gccggtattg caatacccag ctttgactca tatggccatg gaggccgaat tcccggggat 6001 ccgtcgacct gcagccaagc taattccggg cgaatttctt atgatttatg atttttatta 6061 ttaaataagt tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa 6121 aacgaaaatt cttgttcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat 6181 agcatgaggt cgctcttatt gaccacacct ctaccggcat gcaagcttgg cgtaatcatg 6241 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 6301 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg aggtaactca cattaattgc 6361 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgg attaatgaat 6421 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 6481 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 6541 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 6601 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 6661 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 6721 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 6781 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 6841 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 6901 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 6961 cccggtaaga cacgacttat cgccactggc agcagccact agtaacagga ttagcagagc 7021 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 7081 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 7141 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 7201 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 7261 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 7321 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 7381 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 7441 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 7501 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 7561 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 7621 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 7681 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 7741 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 7801 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 7861 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 7921 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 7981 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 8041 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 8101 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 8161 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 8221 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 8281 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 8341 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ct //