LOCUS CVU30497 8392 bp DNA circular SYN 09-APR-1996
DEFINITION Cloning vector pAS2-1, complete sequence.
ACCESSION U30497
VERSION U30497.1
KEYWORDS .
SOURCE Cloning vector pAS2-1
ORGANISM Cloning vector pAS2-1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 8392)
AUTHORS Kitts,P.A.
TITLE CLONTECH Vectors On Disk, version 1.3
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 8392)
AUTHORS Stile,J.S.
TITLE Direct Submission
JOURNAL Submitted (28-JUN-1995) John S. Stile, CLONTECH Laboratories, Inc.,
4030 Fabian Way, Palo Alto, CA 94303, USA
COMMENT On Apr 9, 1996 this sequence version replaced gi:988208.
This vector can be obtained from CLONTECH Laboratories, Inc., 4030
Fabian Way, Palo Alto, CA 94303, USA. To place an order call
(415) 424-8222 or (800) 662-2566, extension 1. International
customers, please contact your local distributor. For technical
information, call (415) 424-8222 or (800) 662-2566, extension 3.
This sequence has been compiled from information in the sequence
databases, published literature and other sources, together with
partial sequences obtained by CLONTECH. If you suspect there is an
error in this sequence, please contact CLONTECH's Technical
Service Department at (415) 424-8222 or (800) 662-2566, extension 3
or E-mail TECH@CLONTECH.COM.
FEATURES Location/Qualifiers
source 1..8392
/organism="Cloning vector pAS2-1"
/mol_type="genomic DNA"
/db_xref="taxon:42098"
rep_origin 1..1348
/note="two micron origin of replication (B form)"
gene 1884..2558
/gene="TRP1"
CDS 1884..2558
/gene="TRP1"
/codon_start=1
/transl_table=11
/protein_id="AAA96381.1"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
VKNAKK"
rep_origin 2609..3277
/note="f1+ origin"
gene complement(3800..4304)
/gene="CYH2"
CDS complement(3855..4304)
/gene="CYH2"
/codon_start=1
/transl_table=11
/protein_id="AAA96382.1"
/translation="MPSRFTKTRKHRGHVSAGKGRIGKHRKHPGGRGMAGGQHHHRIN
MDKYHPGYFGKVGMRYFHKQQAHFWKPVLNLDKLWTLIPEDKRDQYLKSASKETAPVI
DTLAAGYGKILGKGRIPNVPVIVKARFVSKLAEEKIRAAGGVVELIA"
regulatory 4767..5474
/regulatory_class="promoter"
/note="from ADH1 gene"
CDS 5501..6064
/note="contains Gal4 binding domain and epitopes for
monoclonal antibody binding"
/codon_start=1
/transl_table=11
/product="fusion protein"
/protein_id="AAA96383.1"
/translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSPVLQYPALTHMAM
EAEFPGIRRPAAKLIPGEFLMIYDFYY"
misc_feature 5501..5941
/note="encodes Gal4 binding domain"
misc_feature 5952..5969
/note="encodes epitope for monoclonals D11 and F10
binding"
misc_feature 5970..6015
/note="multiple cloning site"
regulatory 6031..6223
/regulatory_class="terminator"
/note="from ADH1 gene; contains stop codons for frame 1,2
and 3"
rep_origin 6231..7402
/note="pUC origin of replication"
gene complement(7402..8262)
/gene="ampicillin resistance"
CDS complement(7402..8262)
/gene="ampicillin resistance"
/codon_start=1
/transl_table=11
/protein_id="AAA96384.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 2351 a 1754 c 1870 g 2417 t
ORIGIN
1 gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt
61 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt
121 ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta
181 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg
241 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag
301 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat
361 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg
421 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact
481 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga
541 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca
601 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac
661 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa
721 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag
781 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag
841 atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt
901 ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg
961 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt
1021 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca
1081 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt
1141 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc
1201 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact
1261 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc
1321 tatcatttcc tttgatattg gatcatatta agaaaccatt attatcatga cattaaccta
1381 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
1441 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
1501 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
1561 tgcggcatca gagcagattg tactgagagt gcaccataga tcaacgacat tactatatat
1621 ataatatagg aagcatttaa tagacagcat cgtaatatat gtgtactttg cagttatgac
1681 gccagatggc agtagtggaa gatattcttt attgaaaaat agcttgtcac cttacgtaca
1741 atcttgatcc ggagcttttc tttttttgcc gattaagaat taattcggtc gaaaaaagaa
1801 aaggagaggg ccaagaggga gggcattggt gactattgag cacgtgagta tacgtgatta
1861 agcacacaaa ggcagcttgg agtatgtctg ttattaattt cacaggtagt tctggtccat
1921 tggtgaaagt ttgcggcttg cagagcacag aggccgcaga atgtgctcta gattccgatg
1981 ctgacttgct gggtattata tgtgtgccca atagaaagag aacaattgac ccggttattg
2041 caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag ttcaggcact ccgaaatact
2101 tggttggcgt gtttcgtaat caacctaagg aggatgtttt ggctctggtc aatgattacg
2161 gcattgatat cgtccaactg catggagatg agtcgtggca agaataccaa gagttcctcg
2221 gtttgccagt tattaaaaga ctcgtatttc caaaagactg caacatacta ctcagtgcag
2281 cttcacagaa acctcattcg tttattccct tgtttgattc agaagcaggt gggacaggtg
2341 aacttttgga ttggaactcg atttctgact gggttggaag gcaagagagc cccgaaagct
2401 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa
2461 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta
2521 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat
2581 ttaagtattg tttgtgcact tgccgatcta tgcggtgtga aataccgcac agatgcgtaa
2641 ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa
2701 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
2761 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
2821 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
2881 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
2941 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
3001 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
3061 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcgcg
3121 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
3181 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
3241 gttttcccag tcacgacgtt gtaaaacgac ggccagtcgt ccaagctttc gcgagctcga
3301 gatcccgagc tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt
3361 tcagtataat gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata
3421 taaataacgt tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag
3481 gttgtctaac tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc
3541 gtgacataac taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt
3601 ttttcaagta ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca
3661 tcttggtact tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
3721 tttttttttt tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga
3781 agtagatgtt gaattagatt aaactgaaga tatataattt attggaaaat acatagagct
3841 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct
3901 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg gattctaccc
3961 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta
4021 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc
4081 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc
4141 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg
4201 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag
4261 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat
4321 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag
4381 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata
4441 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg
4501 cgccagagga aaataggaaa aataacaggg cattagaaaa ataattttga ttttggtaat
4561 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt
4621 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc
4681 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca
4741 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg
4801 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt
4861 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca
4921 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa
4981 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt
5041 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt
5101 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaaat gatggaagac
5161 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga
5221 tgaggggtat cttcgaacac acgaaacttt ttccttcctt cattcacgca cactactctc
5281 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaagttt
5341 gccgctttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat
5401 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag
5461 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat
5521 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa
5581 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc
5641 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct
5701 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca
5761 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt
5821 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat
5881 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc
5941 gccggtattg caatacccag ctttgactca tatggccatg gaggccgaat tcccggggat
6001 ccgtcgacct gcagccaagc taattccggg cgaatttctt atgatttatg atttttatta
6061 ttaaataagt tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa
6121 aacgaaaatt cttgttcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat
6181 agcatgaggt cgctcttatt gaccacacct ctaccggcat gcaagcttgg cgtaatcatg
6241 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
6301 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg aggtaactca cattaattgc
6361 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgg attaatgaat
6421 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
6481 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
6541 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
6601 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
6661 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
6721 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
6781 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
6841 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
6901 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
6961 cccggtaaga cacgacttat cgccactggc agcagccact agtaacagga ttagcagagc
7021 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
7081 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
7141 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
7201 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
7261 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
7321 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
7381 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
7441 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
7501 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
7561 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
7621 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
7681 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
7741 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
7801 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
7861 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
7921 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
7981 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
8041 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
8101 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
8161 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
8221 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
8281 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
8341 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ct
//