LOCUS LT727183 6106 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pEF6/E-Myc-HisA, complete sequence.
ACCESSION LT727183
VERSION LT727183.1
KEYWORDS .
SOURCE Mammalian expression vector pEF6/E-Myc-HisA
ORGANISM Mammalian expression vector pEF6/E-Myc-HisA
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6106)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6106
/organism="Mammalian expression vector pEF6/E-Myc-HisA"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4743."
/db_xref="taxon:1944987"
regulatory 386..1529
/note="hEF1a promoter"
/regulatory_class="promoter"
misc_feature 582..1520
/note="intron hEF1a promoter"
regulatory 1546..1562
/note="T7 promoter"
/regulatory_class="promoter"
misc_feature 1639..1677
/note="E-tag"
CDS <1678..>1936
/codon_start=1
/transl_table=11
/note="mCASP-1 fragment"
/protein_id="SJL87694.1"
/translation="AAADKILRAKRKQFINSVSIGTINGLLDELLEKRVLNQEEMDKI
KLANITAMDKARDLCDHVSKKGPQASQIFITYICNEDCYLAG"
misc_feature 1988..2017
/note="Myc epitope"
misc_feature 2033..2050
/note="His-tag"
regulatory 2079..2303
/note="BGH polyA"
/regulatory_class="terminator"
rep_origin 2349..2777
/note="f1 ori"
regulatory 2782..3063
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 3020..3095
/note="SV40 ori"
regulatory 3168..3233
/note="synthetic EM7 promoter"
/regulatory_class="promoter"
CDS 3234..3632
/transl_table=11
/note="blasticidin resistance"
/protein_id="SJL87695.1"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFT
GVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHP
GIKAIVKDSDGQPTAVGIRELLPSGYVWEG"
regulatory 3790..3864
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(3790..3864)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 4003..4086
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(4178..4569)
/note="pMB1 ori"
CDS complement(5110..5970)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87696.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1395 a 1541 c 1627 g 1543 t
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga
301 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg
361 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
421 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
481 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
541 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
601 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct
661 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
721 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
781 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
841 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
901 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
961 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
1021 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
1081 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
1141 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
1201 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
1261 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
1321 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
1381 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
1441 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
1501 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta
1561 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac tagtccagtg
1621 tggtggaatt ccaccatggg tgcgccggtg ccgtatccgg acccgctgga accgcgtgcg
1681 gccgctgaca agatcctgag ggcaaagagg aagcaattta tcaactcagt gagtataggg
1741 acaataaatg gattgttgga tgaactttta gagaagagag tgctgaatca ggaagaaatg
1801 gataaaataa aacttgcaaa cattactgct atggacaagg cacgggacct atgtgatcat
1861 gtctctaaaa aagggcccca ggcaagccaa atctttatca cttacatttg taatgaagac
1921 tgctacctgg caggaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg
1981 gcccttcgaa caaaaactca tctcagaaga ggatctgaat atgcataccg gtcatcatca
2041 ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
2101 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
2161 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
2221 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
2281 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
2341 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
2401 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
2461 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
2521 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
2581 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
2641 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
2701 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
2761 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
2821 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
2881 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
2941 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
3001 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
3061 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
3121 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat
3181 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca
3241 agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca
3301 tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct
3361 tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg
3421 gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga
3481 acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg
3541 ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat
3601 tgctgccctc tggttatgtg tgggagggct aagcacttcg tggccgagga gcaggactga
3661 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
3721 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
3781 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
3841 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
3901 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
3961 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
4021 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
4081 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
4141 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
4201 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
4261 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
4321 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
4381 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
4441 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
4501 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
4561 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
4621 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
4681 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
4741 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
4801 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
4861 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
4921 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
4981 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
5041 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
5101 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
5161 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
5221 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
5281 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
5341 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
5401 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
5461 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
5521 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
5581 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
5641 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
5701 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
5761 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
5821 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
5881 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
5941 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
6001 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
6061 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//