LOCUS LT727183 6106 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pEF6/E-Myc-HisA, complete sequence. ACCESSION LT727183 VERSION LT727183.1 KEYWORDS . SOURCE Mammalian expression vector pEF6/E-Myc-HisA ORGANISM Mammalian expression vector pEF6/E-Myc-HisA other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 6106) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..6106 /organism="Mammalian expression vector pEF6/E-Myc-HisA" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 4743." /db_xref="taxon:1944987" regulatory 386..1529 /note="hEF1a promoter" /regulatory_class="promoter" misc_feature 582..1520 /note="intron hEF1a promoter" regulatory 1546..1562 /note="T7 promoter" /regulatory_class="promoter" misc_feature 1639..1677 /note="E-tag" CDS <1678..>1936 /codon_start=1 /transl_table=11 /note="mCASP-1 fragment" /protein_id="SJL87694.1" /translation="AAADKILRAKRKQFINSVSIGTINGLLDELLEKRVLNQEEMDKI KLANITAMDKARDLCDHVSKKGPQASQIFITYICNEDCYLAG" misc_feature 1988..2017 /note="Myc epitope" misc_feature 2033..2050 /note="His-tag" regulatory 2079..2303 /note="BGH polyA" /regulatory_class="terminator" rep_origin 2349..2777 /note="f1 ori" regulatory 2782..3063 /note="SV40 early promoter" /regulatory_class="promoter" rep_origin 3020..3095 /note="SV40 ori" regulatory 3168..3233 /note="synthetic EM7 promoter" /regulatory_class="promoter" CDS 3234..3632 /transl_table=11 /note="blasticidin resistance" /protein_id="SJL87695.1" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFT GVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHP GIKAIVKDSDGQPTAVGIRELLPSGYVWEG" regulatory 3790..3864 /note="SV40 polyA early" /regulatory_class="terminator" regulatory complement(3790..3864) /note="SV40 polyA late" /regulatory_class="terminator" regulatory 4003..4086 /note="lac promoter" /regulatory_class="promoter" rep_origin complement(4178..4569) /note="pMB1 ori" CDS complement(5110..5970) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL87696.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 1395 a 1541 c 1627 g 1543 t ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga 301 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg 361 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 421 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 481 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 541 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt 601 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct 661 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga 721 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct 781 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt 841 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc 901 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg 961 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac 1021 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc 1081 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag 1141 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct 1201 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg 1261 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga 1321 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc 1381 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg 1441 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa 1501 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta 1561 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac tagtccagtg 1621 tggtggaatt ccaccatggg tgcgccggtg ccgtatccgg acccgctgga accgcgtgcg 1681 gccgctgaca agatcctgag ggcaaagagg aagcaattta tcaactcagt gagtataggg 1741 acaataaatg gattgttgga tgaactttta gagaagagag tgctgaatca ggaagaaatg 1801 gataaaataa aacttgcaaa cattactgct atggacaagg cacgggacct atgtgatcat 1861 gtctctaaaa aagggcccca ggcaagccaa atctttatca cttacatttg taatgaagac 1921 tgctacctgg caggaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg 1981 gcccttcgaa caaaaactca tctcagaaga ggatctgaat atgcataccg gtcatcatca 2041 ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc 2101 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt 2161 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct 2221 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc 2281 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg 2341 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag 2401 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt 2461 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt 2521 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg 2581 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 2641 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt 2701 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca 2761 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca 2821 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt 2881 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 2941 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc 3001 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct 3061 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa 3121 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat 3181 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca 3241 agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca 3301 tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct 3361 tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg 3421 gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga 3481 acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg 3541 ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat 3601 tgctgccctc tggttatgtg tgggagggct aagcacttcg tggccgagga gcaggactga 3661 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 3721 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 3781 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 3841 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 3901 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg 3961 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 4021 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 4081 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 4141 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 4201 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 4261 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 4321 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 4381 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 4441 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 4501 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 4561 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 4621 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 4681 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 4741 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 4801 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 4861 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 4921 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 4981 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 5041 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 5101 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 5161 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 5221 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 5281 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 5341 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 5401 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 5461 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 5521 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 5581 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 5641 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 5701 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 5761 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 5821 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 5881 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 5941 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 6001 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 6061 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc //