LOCUS Y08011 4626 bp DNA linear SYN 13-NOV-1996
DEFINITION Artificial sequence expression vector pASK85 encoding V regions of
antibody IN-1.
ACCESSION Y08011
VERSION Y08011.1
KEYWORDS IgM; immunoglobulin-kappa; variable region.
SOURCE synthetic construct
ORGANISM synthetic construct
other sequences; artificial sequences.
REFERENCE 1
AUTHORS Bandtlow C., Schiweck W., Tai H.H., Schwab M.M., Skerra A.
TITLE The Escherichia coli-derived Fab fragment of the IgM/kappa antibody
IN-1 recognizes and neutralizes myelin-associated inhibitors of
neurite growth
JOURNAL Eur. J. Biochem. 241(2), 468-475(1996).
PUBMED 8917444
REFERENCE 2 (bases 1 to 4626)
AUTHORS Skerra A.
JOURNAL Submitted (13-SEP-1996) to the INSDC. A. Skerra, Technische
Hochschule Darmstadt, Institut Fuer Biochemie, Petersenstrasse 22,
Darmstadt, D-64287, FRG
FEATURES Location/Qualifiers
source 1..4626
/organism="synthetic construct"
/lab_host="E.coli"
/mol_type="other DNA"
/note="expression vector pASK85-IN1"
/db_xref="taxon:32630"
CDS 147..899
/transl_table=11
/product="immunoglobulin IN-1 heavy chain"
/protein_id="CAA69274.1"
/translation="MKKTAIAIAVALAGFATVAQAEVKLHESGPGLVRPGTSVKISCK
ASGYTFTNYWLGWVKQRPGHGLEWIGDIYPGGGYTNYNEKFKGKATLTADTSSSTAYM
QLSSLTSEDSAVYFCARFYYGSSYWYFDVWGQGTTVTVSSAKTTPPSVYPLAPGSAAQ
TNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWP
SETVTCNVAHPASSTKVDKKIVPRDCHHHHHH"
sig_peptide 147..209
/note="OmpA"
mat_peptide 210..896
V_region 210..572
C_region 573..878
/note="from mouse IgG1"
misc_feature 879..896
/note="(His)6 tag"
CDS 919..1626
/transl_table=11
/transl_except=(pos:919..921,aa:Met)
/product="immunoglobulin IN-1 light chain"
/protein_id="CAA69275.1"
/translation="MKQSTIALALLPLLFTPVTKADIELTQSPAIMAASVGETVTITC
GASENIYGALNWYQRKQGKSPQLLIYGATNLADGMSSRFSGSGSGRQYSLKISSLHPD
DVATYYCQNVLSTPRTFGAGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNF
YPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEAT
HKTSTSPIVKSFNRNEC"
sig_peptide 919..981
/note="PhoA"
mat_peptide 982..1623
V_region 982..1302
C_region 1303..1623
/note="from mouse kappa"
BASE COUNT 1223 a 1117 c 1110 g 1176 t
ORIGIN
1 acccgacacc atcgaatggc cagatgatta attcctaatt tttgttgaca ctctatcatt
61 gatagagtta ttttaccact ccctatcagt gatagagaaa agtgaaatga atagttcgac
121 aaaaatctag ataacgaggg caaaaaatga aaaagacagc tatcgcgatt gcagtggcac
181 tggctggttt cgctaccgta gcgcaggccg aagttaaact gcatgagtca gggcctgggc
241 tggtaaggcc tgggacttca gtgaagatat cctgcaaggc ttctggctac accttcacta
301 actactggct aggttgggta aagcagaggc ctggacatgg acttgagtgg attggagata
361 tttaccctgg aggtggttat actaactaca atgagaagtt caagggcaag gccacactga
421 ctgcagacac atcctccagc actgcctaca tgcagctcag tagcctgaca tctgaggact
481 ctgctgtcta tttctgtgca agattttact acggtagtag ctactggtac ttcgatgtct
541 ggggccaagg caccacggtc accgtctcct cagcaaagac cactcctccg tctgtttacc
601 ctctggctcc tggttctgcg gctcagacta actctatggt gactctggga tgcctggtca
661 agggctattt ccctgagcca gtgacagtga cctggaactc tggatccctg tccagcggtg
721 tgcacacctt cccagctgtc ctgcaatctg acctctacac tctgagcagc tcagtgactg
781 tcccctccag cacctggccc agcgagaccg tcacctgcaa cgttgcccac ccggcttcta
841 gcaccaaagt tgacaagaaa atcgtaccgc gcgactgcca tcaccaccat caccattaat
901 aaccatggag aaaataaagt gaaacaaagc actattgcac tggcactctt accgttactg
961 tttacccctg tgacaaaagc cgacatcgag ctcacccagt ctccagcaat catggctgca
1021 tctgtgggag aaactgtcac catcacatgt ggagcaagtg agaatattta cggtgcttta
1081 aattggtatc agcggaaaca gggaaaatct cctcagctcc tgatctatgg tgcaaccaac
1141 ttggcagatg gcatgtcatc gaggttcagt ggcagtggat ctggtagaca gtattctctc
1201 aagatcagta gcctgcatcc tgacgatgtt gcaacgtatt actgtcaaaa tgtgttaagt
1261 actcctcgga cgttcggagc tgggaccaag ctcgagctga agcgcgctga tgctgcaccg
1321 actgtatcca tcttcccacc atccagtgag cagttaacat ctggaggtgc ctcagtcgtg
1381 tgcttcttga acaacttcta ccccaaagac atcaatgtca agtggaagat tgatggcagt
1441 gaacgacaaa atggcgtcct gaacagttgg actgatcagg acagcaaaga cagcacctac
1501 agcatgagca gcaccctcac gttgaccaag gacgagtatg aacgacataa cagctatacc
1561 tgtgaggcca ctcacaagac atcaacttca cccattgtca agagcttcaa caggaatgag
1621 tgttagagac aaaggtcctg atgctgctga tagcaggtaa gcttgacctg tgaagtgaaa
1681 aatggcgcac attgtgcgac attttttttg tctgccgttt accgctactg cgtcacggat
1741 ctccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
1801 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
1861 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
1921 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
1981 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
2041 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
2101 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
2161 tttaacgcga attttaacaa aatattaacg cttacaattt caggtggcac ttttcgggga
2221 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
2281 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
2341 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
2401 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
2461 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
2521 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
2581 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
2641 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
2701 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
2761 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
2821 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
2881 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
2941 caattgatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
3001 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtggctc tcgcggtatc
3061 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
3121 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
3181 aagcattggt aggaattaat gatgtctcgt ttagataaaa gtaaagtgat taacagcgca
3241 ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag
3301 ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac
3361 gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa
3421 agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat
3481 cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc
3541 gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca
3601 ctcagcgcag tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc
3661 gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct
3721 atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc
3781 atatgcggat tagaaaaaca acttaaatgt gaaagtgggt cttaaaagca gcataacctt
3841 tttccgtgat ggtaacttca ctagtttaaa aggatctagg tgaagatcct ttttgataat
3901 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
3961 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
4021 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
4081 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
4141 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
4201 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
4261 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
4321 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
4381 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
4441 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
4501 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
4561 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
4621 cacatg
//