LOCUS X06403 4245 bp DNA circular SYN 14-NOV-2006
DEFINITION Cloning vector pACYC184.
ACCESSION X06403
VERSION X06403.1
KEYWORDS chloramphenicol resistance; cloning vector; plasmid; tetracycline
resistance.
SOURCE synthetic construct
ORGANISM synthetic construct
other sequences; artificial sequences.
REFERENCE 1 (bases 1 to 4244)
AUTHORS Rose R.E.
JOURNAL Submitted (15-DEC-1987) to the INSDC. Rose R.E., Calgene Inc., 1920
Fifth St., Davis, CA 95616, USA.
REFERENCE 2 (bases 1 to 944;946 to 4245)
AUTHORS Rose R.E.
TITLE The nucleotide sequence of pACYC184
JOURNAL Nucleic Acids Res. 16(1), 355-355(1988).
PUBMED 3340533
REFERENCE 3 (bases 945 to 945)
AUTHORS Mok Y.K., Clark D.R., Kam K.M., Shaw P.C.
TITLE BsiY I, a novel thermophilic restriction endonuclease that
recognizes 5' CCNNNNNNNGG 3' and the discovery of a wrongly
sequenced site in pACYC177
JOURNAL Nucleic Acids Res. 19(9), 2321-2323(1991).
PUBMED 2041772
REFERENCE 4
AUTHORS Li J.K., Tu J.
TITLE Ssp RF1, a novel class-II restriction endonuclease from
Synechococcus RF-1 recognizing 5'TT/CGAA-3'
JOURNAL Nucleic Acids Res. 19(17), 4770-4770(1991).
PUBMED 1909785
COMMENT The sequence is circular, however the sequence begins with a unique
EcoRI site. Bases 426-706, 1153-1583 and 2918 to 3735 were
determined while the rest of the sequence was taken from the
published sequence of pSC101, Tn9 and p15A.
FEATURES Location/Qualifiers
source 1..4245
/organism="synthetic construct"
/plasmid="pACYC184"
/mol_type="other DNA"
/db_xref="taxon:32630"
misc_feature 219..3805
/note="chloramphenicol resistance gene"
misc_feature 443..583
/note="part of IS1 from Tn9"
rep_origin 581..1493
/note="p15A origin of replication"
misc_feature complement(585..3531)
/note="Tn9 HaeII fragment"
misc_difference 944..946
/replace="tc"
/note="conflict"
/citation=[1]
/citation=[2]
misc_feature 1495..3276
/note="pSC101 fragment"
misc_feature 1581..2771
/note="tetracycline resistance gene"
BASE COUNT 956 a 1167 c 1094 g 1028 t
ORIGIN
1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca
1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc
1501 atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct
1561 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg
1621 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg
1681 atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt
1741 tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc
1801 cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac
1861 ccgtcctgtg gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg
1921 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg
1981 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt
2041 tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc
2101 tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga ccgatgccct
2161 tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg
2221 cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg
2281 tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg
2341 tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt
2401 tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc
2461 tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg
2521 gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg
2581 gacagcttca aggatcgctc gcggctctta ccagcctaac ttcgatcact ggaccgctga
2641 tcgtcacggc gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag
2701 gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca
2761 cctcgacctg aatggaagcc ggcggcacct cgctaacgga ttcaccactc caagaattgg
2821 agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc
2881 catcgcgtcc gccatctcca gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg
2941 gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct ggcggggttg
3001 ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg actgctgctg
3061 caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg tttcgtaaag
3121 tctggaaacg cggaagtccc ctacgtgctg ctgaagttgc ccgcaacaga gagtggaacc
3181 aaccggtgat accacgatac tatgactgag agtcaacgcc atgagcggcc tcatttctta
3241 ttctgagtta caacagtccg caccgctgtc cggtagctcc ttccggtggg cgcggggcat
3301 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc
3361 ggcagcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc
3421 gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac
3481 acctacatct gtattaacga agcgctaacc gtttttatca ggctctggga ggcagaataa
3541 atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg gcctcaggca
3601 tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa ccagcaatag
3661 acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa tttgctttcg
3721 aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag gcgtttaagg
3781 gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg
3841 taattcatta agcattctgc cgacatggaa gccatcacag acggcatgat gaacctgaat
3901 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
3961 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
4021 attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
4081 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
4141 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
4201 aacactatcc catatcacca gctcaccgtc tttcattgcc atacg
//