LOCUS R04504 352 bp mRNA linear EST 31-MAR-1995 DEFINITION pk24e10.s1 Kuwabara Mixed stage C. briggsae Caenorhabditis briggsae cDNA similar to SP:BT1_MAIZE P29518 BRITTLE-1 PROTEIN, mRNA sequence. ACCESSION R04504 VERSION R04504.1 DBLINK BioSample: SAMN00154883 KEYWORDS EST. SOURCE Caenorhabditis briggsae ORGANISM Caenorhabditis briggsae Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis. REFERENCE 1 (bases 1 to 352) AUTHORS Hillier,L., Chiapelli,B., Chissoe,S., Clark,N., Couch,J., Dubuque,T., Hawkins,M., Holman,M., Hultman,M., Kucaba,T., Kuwabara,P., Le,M., Mardis,E., Marra,M., Parsons,J., Rifkin,L., Rohlfing,T., Tan,F., Trevaskis,E., Waterston,R., Wohldmann,P. and Wilson,R. TITLE Washington University Caenorhabditis briggsae EST project JOURNAL Unpublished COMMENT Other_ESTs: pk24e10.r1 Contact: Marra MA Washington University Genome Sequencing Center Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1455 Fax: 314 286 1810 Email: mmarra@watson.wustl.edu PCR_F: TGTAAAACGACGGCCAGTGAGCAAGTTCAGCCTGG PCR_B: CAGGAAACAGCTATGACCCTTATGAGTATTTCTTCCAGGGTA Source: Washington University Genome Sequencing Center PCR amplified DNA is available from Washington University Genome Sequencing Center. Aliquots of the library may be requested from P. Kuwabara (pek@mrc-lmb.cam.ac.uk). Seq primer: Commercially available -21M13 dye primer. FEATURES Location/Qualifiers source 1..352 /organism="Caenorhabditis briggsae" /mol_type="mRNA" /strain="G16 Gujarat" /db_xref="taxon:6238" /clone_lib="SAMN00154883 Kuwabara Mixed stage C. briggsae" /note="Vector: Lambda gt10; Site_1: EcoRI; Site_2: EcoRI; Stage:mixed, Sex:hermaphrodite. Library construction: First strand oligo(dT) primed. Second strand was as per Gubler/Hoffman. Ligated to EcoRI adaptors. Library is non-directional. Library is non-normalized. Library constructed by P.E. Kuwabara. Additional details on construction of the library are described in P.E. Kuwabara and S. Shah, NAR 22: 4414 - 4418 (1994). Adaptor sequence: GAATTC CGTTGCTGTCG" BASE COUNT 122 a 73 c 63 g 93 t ORIGIN 1 aaacaataaa aaaacttttn tgaaaagaga actgaacgag aaaataagca tgaaaagagt 61 aactaatgtg aaatatttgt gaaatattgc tgatctacat gaactatgag tagaaattaa 121 actctagatc ctttttctaa tccaagaatt ttctctgcca atccgccgta ataactgtct 181 gcgcgattcg aacagtggcg catcaccatc atacggcaaa tggctccctt aaacaaagcc 241 ttaacgcctt catccatcca gattcgataa aatgcgtggc aaattccatt gtagttggag 301 cctccagatt ggattctagt tttcacgaca tccacggggt gaccgcaaat ga //