LOCUS       R04504                   352 bp    mRNA    linear   EST 31-MAR-1995
DEFINITION  pk24e10.s1 Kuwabara Mixed stage C. briggsae Caenorhabditis briggsae
            cDNA similar to SP:BT1_MAIZE P29518 BRITTLE-1 PROTEIN, mRNA
            sequence.
ACCESSION   R04504
VERSION     R04504.1
DBLINK      BioSample: SAMN00154883
KEYWORDS    EST.
SOURCE      Caenorhabditis briggsae
  ORGANISM  Caenorhabditis briggsae
            Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
            Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
            Caenorhabditis.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Hillier,L., Chiapelli,B., Chissoe,S., Clark,N., Couch,J.,
            Dubuque,T., Hawkins,M., Holman,M., Hultman,M., Kucaba,T.,
            Kuwabara,P., Le,M., Mardis,E., Marra,M., Parsons,J., Rifkin,L.,
            Rohlfing,T., Tan,F., Trevaskis,E., Waterston,R., Wohldmann,P. and
            Wilson,R.
  TITLE     Washington University Caenorhabditis briggsae EST project
  JOURNAL   Unpublished
COMMENT     Other_ESTs: pk24e10.r1
            Contact: Marra MA
            Washington University Genome Sequencing Center
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1455
            Fax: 314 286 1810
            Email: mmarra@watson.wustl.edu
            PCR_F: TGTAAAACGACGGCCAGTGAGCAAGTTCAGCCTGG
            PCR_B: CAGGAAACAGCTATGACCCTTATGAGTATTTCTTCCAGGGTA
            Source: Washington University Genome Sequencing Center
            PCR amplified DNA is available from Washington University Genome
            Sequencing Center. Aliquots of the library may be requested from P.
            Kuwabara (pek@mrc-lmb.cam.ac.uk).
            Seq primer: Commercially available -21M13 dye primer.
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Caenorhabditis briggsae"
                     /mol_type="mRNA"
                     /strain="G16 Gujarat"
                     /db_xref="taxon:6238"
                     /clone_lib="SAMN00154883 Kuwabara Mixed stage C. briggsae"
                     /note="Vector: Lambda gt10; Site_1: EcoRI; Site_2: EcoRI;
                     Stage:mixed, Sex:hermaphrodite. Library construction:
                     First strand oligo(dT) primed. Second strand was as per
                     Gubler/Hoffman. Ligated to EcoRI adaptors. Library is
                     non-directional. Library is non-normalized. Library
                     constructed by P.E. Kuwabara. Additional details on
                     construction of the library are described in P.E. Kuwabara
                     and S. Shah, NAR 22: 4414 - 4418 (1994). Adaptor sequence:
                     GAATTC CGTTGCTGTCG"
BASE COUNT          122 a           73 c           63 g           93 t
ORIGIN      
        1 aaacaataaa aaaacttttn tgaaaagaga actgaacgag aaaataagca tgaaaagagt
       61 aactaatgtg aaatatttgt gaaatattgc tgatctacat gaactatgag tagaaattaa
      121 actctagatc ctttttctaa tccaagaatt ttctctgcca atccgccgta ataactgtct
      181 gcgcgattcg aacagtggcg catcaccatc atacggcaaa tggctccctt aaacaaagcc
      241 ttaacgcctt catccatcca gattcgataa aatgcgtggc aaattccatt gtagttggag
      301 cctccagatt ggattctagt tttcacgaca tccacggggt gaccgcaaat ga
//