LOCUS R04028 336 bp mRNA linear EST 31-MAR-1995 DEFINITION pk16h01.r1 Kuwabara Mixed stage C. briggsae Caenorhabditis briggsae cDNA similar to SP:SYS_THETH P34945 SERYL-TRNA SYNTHETASE, mRNA sequence. ACCESSION R04028 VERSION R04028.1 DBLINK BioSample: SAMN00154883 KEYWORDS EST. SOURCE Caenorhabditis briggsae ORGANISM Caenorhabditis briggsae Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis. REFERENCE 1 (bases 1 to 336) AUTHORS Hillier,L., Chiapelli,B., Chissoe,S., Clark,N., Couch,J., Dubuque,T., Hawkins,M., Holman,M., Hultman,M., Kucaba,T., Kuwabara,P., Le,M., Mardis,E., Marra,M., Parsons,J., Rifkin,L., Rohlfing,T., Tan,F., Trevaskis,E., Waterston,R., Wohldmann,P. and Wilson,R. TITLE Washington University Caenorhabditis briggsae EST project JOURNAL Unpublished COMMENT Other_ESTs: pk16h01.s1 Contact: Marra MA Washington University Genome Sequencing Center Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1455 Fax: 314 286 1810 Email: mmarra@watson.wustl.edu PCR_F: TGTAAAACGACGGCCAGTGAGCAAGTTCAGCCTGG PCR_B: CAGGAAACAGCTATGACCCTTATGAGTATTTCTTCCAGGGTA Source: Washington University Genome Sequencing Center Putatively trans-spliced (TSL site SL1). PCR amplified DNA is available from Washington University Genome Sequencing Center. Aliquots of the library may be requested from P. Kuwabara (pek@mrc-lmb.cam.ac.uk). Seq primer: Commercially available M13 reverse dye primer. FEATURES Location/Qualifiers source 1..336 /organism="Caenorhabditis briggsae" /mol_type="mRNA" /strain="G16 Gujarat" /db_xref="taxon:6238" /clone_lib="SAMN00154883 Kuwabara Mixed stage C. briggsae" /note="Vector: Lambda gt10; Site_1: EcoRI; Site_2: EcoRI; Stage:mixed, Sex:hermaphrodite. Library construction: First strand oligo(dT) primed. Second strand was as per Gubler/Hoffman. Ligated to EcoRI adaptors. Library is non-directional. Library is non-normalized. Library constructed by P.E. Kuwabara. Additional details on construction of the library are described in P.E. Kuwabara and S. Shah, NAR 22: 4414 - 4418 (1994). Adaptor sequence: GAATTC CGTTGCTGTCG" BASE COUNT 79 a 72 c 77 g 107 t ORIGIN 1 agagattttg ccnggctgga aacgaagggg ccgccacgtt gcttaattct cttccgaagg 61 gatctggaag ctctccaagt ccttttttgt cattttgaaa cgtttcaaga actgatataa 121 gtgttcttgt tgaagaaagg gctgttccgt tgcaagtgtg gacatatttg gttgtgccgt 181 cagcagtctt gtatttgata ccaagtcttc ttgcttgaaa atccgtgcaa ttactggctg 241 aagaaacctc tccatacagt ttccttccag gcatccatgc ttcgatatcg aattttttag 301 ccagcagatg cgccaagttc ttccaggaag gcattt //